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Anti-osteogenic activity of cadmium in zebrafish

2019Journal article Open access
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dc.contributor.authorTarasco, Marco
dc.contributor.authorCardeira Da Silva, João
dc.contributor.authorViegas, Michael
dc.contributor.authorCaria, Joana
dc.contributor.authorMartins, Gil
dc.contributor.authorGavaia, Paulo
dc.contributor.authorCancela, M. Leonor
dc.contributor.authorLaizé, Vincent
dc.date.accessioned2020-07-29T11:13:57Z
dc.date.available2020-07-29T11:13:57Z
dc.date.issued2019
dc.description.abstractAmong the many anthropogenic chemicals that end up in the aquatic ecosystem, heavy metals, in particular cadmium, are hazardous compounds that have been shown to affect developmental, reproductive, hepatic, hematological, and immunological functions in teleost fish. There is also evidence that cadmium disturbs bone formation and skeletal development, but data is scarce. In this work, zebrafish was used to further characterize the anti-osteogenic/osteotoxic effects of cadmium and gain insights into underlying mechanisms. Upon exposure to cadmium, a reduction of the opercular bone growth was observed in 6-days post-fertilization (dpf) larvae and an increase in the incidence of skeletal deformities was evidenced in 20-dpf post-larvae. The extent and stiffness of newly formed bone was also affected in adult zebrafish exposed to cadmium while regenerating their caudal fin. A pathway reporter assay revealed a possible role of the MTF-1 and cAMP/PKA signaling pathways in mechanisms of cadmium osteotoxicity, while the expression of genes involved in osteoblast differentiation and matrix production was strongly reduced in cadmium-exposed post-larvae. This work not only confirmed cadmium anti-osteogenic activity and identified targeted pathways and genes, but it also suggested that cadmium may affect biomechanical properties of bone.pt_PT
dc.description.sponsorshipPortuguese Science and Technology Foundation (FCT) - UID/Multi/04326/2019 (CCMAR), PEst-C/MAR/LA0015/2011 (CCMAR); SFRH/BD/128634/2017 and SFRH/BD/52425/2013pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3390/fishes4010011pt_PT
dc.identifier.issn2410-3888
dc.identifier.urihttp://hdl.handle.net/10400.1/14538
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationAQUATOX - Assessment of mineralogenic toxicity of aquatic pollutants
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectOsteotoxicitypt_PT
dc.subjectZebrafish Danio reriopt_PT
dc.subjectOperculum growthpt_PT
dc.subjectSkeletal deformitiespt_PT
dc.subjectCaudal fin regenerationpt_PT
dc.subjectSignaling pathwayspt_PT
dc.subjectGene expressionpt_PT
dc.titleAnti-osteogenic activity of cadmium in zebrafishpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAQUATOX - Assessment of mineralogenic toxicity of aquatic pollutants
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR%2F112992%2F2009/PT
oaire.citation.issue1pt_PT
oaire.citation.startPage11pt_PT
oaire.citation.titleFishespt_PT
oaire.citation.volume4pt_PT
oaire.fundingStream3599-PPCDT
person.familyNameTarasco
person.familyNameCardeira da Silva
person.familyNameViegas
person.familyNameCaria
person.familyNameMartins
person.familyNameGavaia
person.familyNameCancela
person.familyNameLaizé
person.givenNameMarco
person.givenNameJoão
person.givenNameMichael
person.givenNameJoana
person.givenNameGil
person.givenNamePaulo
person.givenNameM. Leonor
person.givenNameVincent
person.identifier.ciencia-id2B1F-0392-9ABE
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person.identifier.orcid0000-0002-8791-4766
person.identifier.orcid0000-0002-1344-2954
person.identifier.orcid0000-0002-9582-1957
person.identifier.orcid0000-0003-3114-6662
person.identifier.orcid0000-0001-9565-9198
person.identifier.ridG-9575-2017
person.identifier.ridA-6470-2011
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id57194105105
person.identifier.scopus-author-id55224020100
person.identifier.scopus-author-id36505309900
person.identifier.scopus-author-id6507104377
person.identifier.scopus-author-id6602982778
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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