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Dextran sulfate microparticles encapsulating isoniazid and/or rifabutin as carriers for pulmonary tuberculosis therapy

2017-08Conference object Restricted access
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dc.contributor.authorMusacchio, Flavia
dc.contributor.authorGrenha, Ana
dc.date.accessioned2019-11-20T15:07:28Z
dc.date.available2019-11-20T15:07:28Z
dc.date.issued2017-08
dc.description.abstractTuberculosis (TB) is an active deadly pathology with high prevalence worldwide, occurring upon infection with Mycobacterium tuberculosis (MTB). Although conventional oral therapy is effective, it is associated with severe side-effects. The need to overcome these effects, which often lead to patient incompliance, demands alternative therapies, either by using new drugs or exploring new routes of administration of traditional drugs. The latter approach has been gaining popularity, as it could permit the reduction of used doses and the frequency of administration. Using the lung route would provide direct administration of antibiotics to the alveoli, where macrophages hosting the bacillus are located, which potentially comprises a successful approach. This study proposes the establishment of inhalable therapy, using dextran sulfate microparticles as carries of antitubercular drugs. This polysaccharide has structural residues/groups reported to be recognised in a preferential manner by alveolar macrophages, which increases the potential of the system in tuberculosis treatment. Microparticles were produced by spray-drying and characterized for morphology (SEM), Feret’s diameter, bulk and tap densities. The theoretical aerodynamic diameter (daer) was further calculated. The cytotoxic evaluation was performed in alveolar epithelial cells (A549) by the MTT assay. Microparticles were obtained with sizes varying within 1.1 and 1.9 μm, which potentiates phagocytosis. Theoretical daer between 1 and 1.4 μm suggests the adequacy of the carriers for the purpose of reaching the alveolar zone. The cytotoxic evaluation evidenced absence of toxicity. The generality of results provided encouraging indications to continue studying the potential of dextran sulfate microparticles as inhalable tuberculosis therapy.
dc.identifier.issn1941-2711
dc.identifier.issn1941-2703
dc.identifier.urihttp://hdl.handle.net/10400.1/13052
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMary Ann Liebert, Inc
dc.relationFighting TB: Development of microparticulate systems to target alveolar macrophages in tuberculosis therapy
dc.titleDextran sulfate microparticles encapsulating isoniazid and/or rifabutin as carriers for pulmonary tuberculosis therapy
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleFighting TB: Development of microparticulate systems to target alveolar macrophages in tuberculosis therapy
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FDTP-FTO%2F0094%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIM%2F04773%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.citation.endPageA21
oaire.citation.issue4
oaire.citation.startPageA20
oaire.citation.titleJournal of Aerosol Medicine and Pulmonary Drug Delivery
oaire.citation.volume30
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNameGrenha
person.givenNameAna
person.identifier.ciencia-id091C-0D58-7225
person.identifier.orcid0000-0002-2136-1396
person.identifier.ridH-1392-2017
person.identifier.scopus-author-id8607930100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typeconferenceObject
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