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Cytotoxicity of Cryptosula zavjalovensis Kubanin extract against Breast Cancer Cell Line MCF7

2021Journal article Open Access
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dc.contributor.authorGonzaga, Loveille Jun
dc.contributor.authorCavaco, Isabel
dc.contributor.authorFortunato, Helena
dc.date.accessioned2021-09-15T13:29:03Z
dc.date.available2021-09-15T13:29:03Z
dc.date.issued2021
dc.description.abstractThe marine environment is an abundant source of diverse biologically active compounds that demonstrate great potential applications in pharmaceutics and medicine. Although novel biologically active secondary metabolites can be potentially found in bryozoans, there have been a few studies on these organisms. Bryozoans are sessile colonial animals commonly found in great diversity in shallow waters. In this study, samples of the bryozoan Cryptosula zavjalovensis Kubanin were collected from Akkeshi, Japan, and were extracted using ethanol. The crude extract was separated using ethyl acetate (EtOAc) and water to obtain organic and aqueous fractions, respectively. In the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the EtOAc fraction demonstrated cytotoxicity towards MCF7 breast cancer cells. The EtOAc extract was subsequently fractionated through solid-phase extraction using a gradient of methanol and water (E1 80:20 v/v and E2 100:0 v/v) and using methanol and chloroform (E3 50:50 v/v). Toxicity profiling revealed that the toxicity toward the human MCF7 breast cancer cells of the E2 and E3 fractions is comparable to that of cisplatin, indicating the excellent cytotoxic activity of the EtOAc fractions of C. zavjalovensis. Further studies are thus warranted to isolate the novel compounds in these fractions and determine their potential chemotherapeutic application.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.32383/appdr/139634pt_PT
dc.identifier.issn2353-5288
dc.identifier.urihttp://hdl.handle.net/10400.1/17118
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherPolskie Towarzystwo Farmaceutycznept_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectCytotoxicitypt_PT
dc.subjectMarine bryozoanpt_PT
dc.subjectCryptosula zavjalovensispt_PT
dc.subjectMCF7pt_PT
dc.titleCytotoxicity of Cryptosula zavjalovensis Kubanin extract against Breast Cancer Cell Line MCF7pt_PT
dc.title.alternativeCytotoxicidade da criptosula zavjalovensis kubanin extrato contra a linha de células cancerígenas de mama mcf7pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage384pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage379pt_PT
oaire.citation.titleActa Poloniae Pharmaceutica - Drug Researchpt_PT
oaire.citation.volume78pt_PT
person.familyNamePALMA ANTUNES CAVACO
person.givenNameISABEL MARIA
person.identifierH-9087-2012
person.identifier.ciencia-idD515-FB5E-1205
person.identifier.orcid0000-0001-8651-9054
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb499959b-bea5-4292-bdb2-4311b0aa82de
relation.isAuthorOfPublication.latestForDiscoveryb499959b-bea5-4292-bdb2-4311b0aa82de

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