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Cloning and expression of a GST-pi gene in Mytilus galloprovincialis. Attempt to use the GST-pi transcript as a biomarker of pollution

2006Journal article Restricted access
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dc.contributor.authorHoarau, Pascal
dc.contributor.authorDamiens, Gautier
dc.contributor.authorRoméo, Michèle
dc.contributor.authorGnassia-Barelli, Mauricette
dc.contributor.authorBebianno, Maria João
dc.date.accessioned2020-05-11T10:07:07Z
dc.date.available2020-05-11T10:07:07Z
dc.date.issued2006
dc.description.abstractGlutathione S-transferase is involved in the detoxication of many chemical compounds. Northern blot analysis of mRNA GST gene from the mussel Mytilus galloprovincialis shows the presence of a transcript of 850 bp (GenBank accession no. Gi:22094808 and AF527010). The cDNA cloned sequence is constituted by an ORF of 621 bp encoding for a protein of 23,700 Da present in the gills and digestive gland of M galloprovincialis. The sequence, called Mg (M. galloprovincialis) GST-p, is clearly related to the pi class GST. M. galloprovincialis treated with Cd (200 microg/L) and BaP (100 microg/L) or co-treated with Cd and BaP take up cadmium in the gills (16.2 +/- 4.2 and 12.6 +/- 1.2 microg Cd/g dry wt. after exposure to Cd and Cd + BaP, respectively) and in the digestive gland. The transcription of GST-pi gene, by semi-quantitative RT-PCR, shows the lowest value in the digestive glands of mussels exposed to BaP, whereas the treatment with cadmium and the co-treatment with cadmium and BaP evoke GST-pi gene expression higher than controls. Mussels collected from six sites along the south coast of Portugal show different GST-pi transcription levels, some of which are related to their pollutant content.pt_PT
dc.description.sponsorshipPortuguese Foundation for Science and Technologypt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.cbpc.2006.02.007pt_PT
dc.identifier.issn1532-0456
dc.identifier.urihttp://hdl.handle.net/10400.1/13869
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnimalspt_PT
dc.subjectBenzo(a)pyrenept_PT
dc.subjectBiomarkerspt_PT
dc.subjectCadmiumpt_PT
dc.subjectCloningpt_PT
dc.subjectDigestive systempt_PT
dc.subjectEnvironmental monitoringpt_PT
dc.subjectGillspt_PT
dc.subjectGlutathione S-transferase pipt_PT
dc.subjectMolecular sequence datapt_PT
dc.subjectMytiluspt_PT
dc.subjectPhylogenypt_PT
dc.subjectPortugalpt_PT
dc.subjectRNApt_PT
dc.subjectTranscriptionpt_PT
dc.subjectWater pollutantspt_PT
dc.subjectEnvironmental exposurept_PT
dc.subjectGene expression regulationpt_PT
dc.subjectChemicalpt_PT
dc.subjectMessengerpt_PT
dc.subjectGeneticpt_PT
dc.subjectEnzymologicpt_PT
dc.titleCloning and expression of a GST-pi gene in Mytilus galloprovincialis. Attempt to use the GST-pi transcript as a biomarker of pollutionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage203pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage196pt_PT
oaire.citation.titleComparative Biochemistry and Physiology - Part C: Toxicology and Pharmacologypt_PT
oaire.citation.volume143pt_PT
person.familyNameBebianno
person.givenNameMaria
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0003-1492-8566
person.identifier.scopus-author-id7004152715
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isAuthorOfPublication.latestForDiscovery2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32

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