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Serum-specific stimulation of proliferation and mineralization of fish bone-derived cells

2010Journal article Restricted access
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dc.contributor.authorRosa, Joana
dc.contributor.authorTiago, Daniel
dc.contributor.authorDias, J.
dc.contributor.authorCancela, Leonor
dc.contributor.authorLaizé, Vincent
dc.date.accessioned2014-06-17T13:10:28Z
dc.date.available2014-06-17T13:10:28Z
dc.date.issued2010
dc.date.updated2014-06-03T09:47:39Z
dc.description.abstractTeleost fish have recently been implemented as suitable model organisms to study vertebrate development, in particular skeletogenesis. In vitro cell systems derived from fish bone have been successfully established, although their development has been hampered by the limited availability of fish serum to supplement culture medium. Commercially available sera are mostly of mammalian origin and thus not necessarily adequate to fish cell growth. The main objective of this work was to compare proliferative and mineralogenic potential of bovine and fish sera using fish bone-derived cell lines VSa13 and VSa16. Fish serum was shown to (i) strongly stimulate cell proliferation in an apparent dose-dependent and cell type-specific manner, (ii) induce morphological changes, and (iii) enhance extracellular matrix mineralization of bone cells, although cytotoxic for fish osteoblast-like cells at the concentration tested. To better understand mechanisms underlying mineralogenic effect of fish serum in fish chondrocytes, expression of several mineralization-related genes was evaluated by qPCR. Regulation of matrix Gla protein (MGP) and bone morphogenetic protein 2 (BMP2) gene expression was modified upon culture with fish serum in a way compatible with an early onset and an increase in mineralization. In conclusion, fish serum was shown to be more adequate to proliferation and differentiation/mineralization of fish bone-derived cells.por
dc.identifier.citationRosa, J.; Tiago, D.M.; Dias, J.; Cancela, M.L.; Laizé, V.Serum-specific stimulation of proliferation and mineralization of fish bone-derived cells, Journal of Applied Ichthyology, 26, 2, 251-256, 2010.por
dc.identifier.doihttp://dx.doi.org/10.1111/j.1439-0426.2010.01414.x
dc.identifier.issn0175-8659
dc.identifier.otherAUT: LCA00739;
dc.identifier.urihttp://hdl.handle.net/10400.1/4339
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWileypor
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1111/j.1439-0426.2010.01414.x/abstractpor
dc.subjectBone-derived cellspor
dc.subjectSerum-specificpor
dc.titleSerum-specific stimulation of proliferation and mineralization of fish bone-derived cellspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage256por
oaire.citation.issue2por
oaire.citation.startPage251por
oaire.citation.titleJournal of Applied Ichthyologypor
oaire.citation.volume26por
person.familyNameRosa
person.familyNameTiago
person.familyNameCancela
person.familyNameLaizé
person.givenNameJoana
person.givenNameDaniel
person.givenNameM. Leonor
person.givenNameVincent
person.identifier.ciencia-id0B18-8AEC-875C
person.identifier.orcid0000-0001-7947-2681
person.identifier.orcid0000-0001-8418-6292
person.identifier.orcid0000-0003-3114-6662
person.identifier.orcid0000-0001-9565-9198
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id36505067900
person.identifier.scopus-author-id14422711000
person.identifier.scopus-author-id6602982778
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication181f1017-5086-40dc-9edc-a289aaa2d263
relation.isAuthorOfPublication10cda3a0-7b9c-4d16-bd2a-a06df1d56d94
relation.isAuthorOfPublicationb9bbfe32-3dfe-4131-ad14-a4394008447f
relation.isAuthorOfPublicationb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f
relation.isAuthorOfPublication.latestForDiscoveryb51cd3d5-7ee4-47d8-9bc4-b3e297aa222f

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