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An artificial selection procedure enriches for known and suspected chitin degraders from the prokaryotic rare biosphere of multiple marine biotopes

2025-11-25Journal article Open access
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dc.contributor.authorMeunier, Laurence
dc.contributor.authorKeller-Costa, Tina
dc.contributor.authorCannella, David
dc.contributor.authorGonçalves, Jorge Manuel Santos
dc.contributor.authorDechamps, Etienne
dc.contributor.authorMarques, Matilde
dc.contributor.authorCosta, Rodrigo
dc.contributor.authorGeorge, Isabelle F.
dc.date.accessioned2025-12-22T12:59:57Z
dc.date.available2025-12-22T12:59:57Z
dc.date.issued2025-11-25
dc.description.abstractBiological of chitin-degrading microbial communities change across marine biotopes, but efforts to isolate chitin degraders within these communities in the laboratory have seldom been attempted. We characterized the prokaryotic communities associated with the marine sponge Sarcotragus spinosulus, the octocoral Eunicella labiata, and their surrounding sediment and seawater and applied an artificial selection procedure to enrich bacterial consortia capable of degrading chitin from the abovementioned biotopes. Throughout the procedure, chitin degradation was monitored, and the taxonomic composition was studied along four successive enrichment cultures from each biotope. Results The naturally occurring prokaryotic communities of the two host species (Sarcotragus spinosulus and Eunicella labiata) were distinct from each other and from those of seawater and sediments, even though they were co-inhabiting the same geographic area. We found that low-abundance bacteria from the rare biosphere were recruited in the enrichment cultures from all biotopes, while dominant bacterial symbionts likely to play a role in chitin degradation within marine sponges and octocorals remained “unculturable” under our experimental conditions. Well-known chitin degraders such as Vibrio, Pseudoalteromonas and Aquimarina, as well as other taxa not known or poorly known for their role(s) in chitin degradation such as Aureivirga, Halodesulfovibrio, Motilimonas, Muricauda, Psychromonas, Poseidonibacter, Reichenbachiella, and Thalassotalea, among others, were enriched using our artificial selection approach. Distinct chitin-degrading consortia were enriched from each marine biotope, highlighting the feasibility of this approach in fostering the discovery of novel microorganisms and enzymes involved in chitin degradation pathways of relevance in applied biotechnology. Conclusion This study unveils distinct bacterial consortia possessing moderate to high efficiency at degrading chitin. They were composed of a mix of known chitin degraders, known chitin utilizers and many taxa poorly or not yet known for their role(s) in chitin degradation such as Aureivirga, Psychromonas, Motilimonas, Reichenbachiella, or Halodesulfovibrio. The latter taxa are potential key players in marine chitin degradation whose study could lead to the discovery of novel enzyme variants able to degrade chitin and its derivatives.eng
dc.description.sponsorshipCEECIND/00788/2017; SFRH/BD/151376/2021
dc.identifier.doi10.1186/s12866-025-04218-7
dc.identifier.issn1471-2180
dc.identifier.urihttp://hdl.handle.net/10400.1/28008
dc.language.isoeng
dc.peerreviewedyes
dc.publisherBioMed Central
dc.relationHarnessing the catalyst power of host-microbe interactions: a quest for novel chitinases from octocoral symbionts
dc.relationInstitute for Bioengineering and Biosciences
dc.relationInstitute for Bioengineering and Biosciences
dc.relationInstitute for Health and Bioeconomy
dc.relation.ispartofBMC Microbiology
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectProkaryotic communities
dc.subjectEnrichment cultures
dc.subjectChitinase
dc.subjectMarine sponge
dc.subjectOctocoral
dc.subjectSize exclusion chromatography
dc.titleAn artificial selection procedure enriches for known and suspected chitin degraders from the prokaryotic rare biosphere of multiple marine biotopeseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHarnessing the catalyst power of host-microbe interactions: a quest for novel chitinases from octocoral symbionts
oaire.awardTitleInstitute for Bioengineering and Biosciences
oaire.awardTitleInstitute for Bioengineering and Biosciences
oaire.awardTitleInstitute for Health and Bioeconomy
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/EXPL%2FBIA-MIC%2F0286%2F2021/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04565%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04565%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0140%2F2020/PT
oaire.citation.issue1
oaire.citation.titleBMC Microbiology
oaire.citation.volume25
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameGonçalves
person.givenNameJorge Manuel Santos
person.identifier356846
person.identifier.ciencia-id1013-ED6A-65EB
person.identifier.orcid0000-0001-7704-8190
person.identifier.ridG-5206-2011
person.identifier.scopus-author-id7103326116
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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