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Carotenoid biosynthetic gene expression, pigment and n-3 fatty acid contents in carotenoid-rich Tetraselmis striata CTP4 strains under heat stress combined with high light

2021Journal article Restricted access
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dc.contributor.authorSchüler, Lisa Maylin
dc.contributor.authorBombo, Gabriel
dc.contributor.authorDuarte, Paulo
dc.contributor.authorSantos, Tamara
dc.contributor.authorMaia, Inês Beatriz
dc.contributor.authorPinheiro, Filipa
dc.contributor.authorMarques, José
dc.contributor.authorJacinto, Rita
dc.contributor.authorSchulze, Peter S.C.
dc.contributor.authorPereira, Hugo
dc.contributor.authorBarreira, Luísa
dc.contributor.authorVarela, João
dc.date.accessioned2021-09-23T12:36:40Z
dc.date.available2023-06-01T00:30:13Z
dc.date.issued2021
dc.description.abstractIn this study, two carotenoid-rich strains of the euryhaline microalga Tetraselmis striata CTP4 were isolated by random mutagenesis combined with selection via fluorescence activated cell sorting and growth on norflurazon. Both strains, ED5 and B11, showed an up to 1.5-fold increase in carotenoid contents as compared with the wildtype, independent of the growth conditions. More specifically, violaxanthin, beta-carotene and lutein contents reached as high as 1.63, 4.20 and 3.81 mg g-1 DW, respectively. Genes coding for phytoene synthase, phytoene desaturase, lycopene-beta-cyclase and epsilon-ring hydroxylase involved in carotenoid biosynthesis were found to be upregulated in ED5 and B11 cells as compared to the wildtype. Both strains showed higher contents of eicosapentaenoic acid as compared with those of the wildtype, reaching up to 4.41 and 2.88 mg g-1 DW, respectively. Overall, these results highlight the complexity of changes in carotenoid biosynthesis regulation that are required to improve pigment contents in microalgae.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.biortech.2021.125385pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.1/17152
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.subjectMicroalgaept_PT
dc.subjectRandom mutagenesispt_PT
dc.subjectFlow cytometrypt_PT
dc.subjectLuteinpt_PT
dc.subjectEPApt_PT
dc.titleCarotenoid biosynthetic gene expression, pigment and n-3 fatty acid contents in carotenoid-rich Tetraselmis striata CTP4 strains under heat stress combined with high lightpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/62193/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/71879/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/157544/PT
oaire.citation.startPage125385pt_PT
oaire.citation.titleBioresource Technologypt_PT
oaire.citation.volume337pt_PT
oaire.fundingStreamOE
oaire.fundingStreamOE
oaire.fundingStream6817 - DCRRNI ID
person.familyNameSchüler
person.familyNameSantos
person.familyNameMaia
person.familyNameJacinto
person.familyNameSchulze
person.familyNamePereira
person.familyNameBarreira
person.familyNameVarela
person.givenNameLisa
person.givenNameTamara
person.givenNameInês Beatriz
person.givenNameRita
person.givenNamePeter S.C.
person.givenNameHugo
person.givenNameLuísa
person.givenNameJoão
person.identifier2641191
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person.identifier.orcid0000-0003-0961-5397
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person.identifier.orcid0000-0002-0504-6388
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person.identifier.orcid0000-0002-1369-2099
person.identifier.orcid0000-0002-4077-855X
person.identifier.orcid0000-0003-3101-693X
person.identifier.ridJ-9270-2014
person.identifier.ridE-9360-2015
person.identifier.ridM-4223-2013
person.identifier.scopus-author-id56244247100
person.identifier.scopus-author-id54080259900
person.identifier.scopus-author-id55741416000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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