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Changes in metallothionein transcription levels in the mussel Mytilus galloprovincialis exposed to CdTe quantum dots

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dc.contributor.authorLopes Rocha, Thiago
dc.contributor.authorBilbao, Eider
dc.contributor.authorCardoso, Cátia
dc.contributor.authorSoto, Manu
dc.contributor.authorBebianno, Maria João
dc.date.accessioned2020-04-06T13:08:09Z
dc.date.available2020-04-06T13:08:09Z
dc.date.issued2018
dc.description.abstractQuantum dots (QDs) are a class of engineered nanoparticles (ENPs) with several biomedical, industrial and commercial applications. However, their metabolism and detoxification process in aquatic invertebrates and environmental health hazards remain unclear. This study investigate the transcriptional changes of metallothioneins (MTs) isoforms (mt10IIIa and mt20IV) induced by CdTe QDs, in comparison with its dissolved counterpart, in the marine mussel Mytilus galloprovincialis. Mussels were exposed to CdTe QDs and to the same Cd concentration (10 µg Cd L-1) of dissolved Cd for 14 days and mt transcription levels were measured by real time quantitative PCR (qPCR). Tissue specific mt transcription patterns were observed in mussels exposed to both Cd forms, wherein the gills were a more sensitive organ compared to the digestive gland. No significant changes were observed in mt10IIIa transcription levels in mussels exposed to both Cd forms. In contrast, transcription of mt20IV was tissue and exposure time dependent, with higher mt20IV mRNA levels in mussels exposed to QDs and dissolved Cd when compared to unexposed mussels. Multivariate analysis indicates particle-specific effects after 14 days of exposure and a dual role of MTs in the QD metabolism and in the protection against oxidative stress in mussels exposed to Cd-based ENPs.pt_PT
dc.description.sponsorship239524/2012-8pt_PT
dc.description.sponsorship239524/2012
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doihttps:/doi,org/10.1007/s10646-018-1903-ypt_PT
dc.identifier.issn0963-9292
dc.identifier.urihttp://hdl.handle.net/10400.1/13695
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Verlagpt_PT
dc.relationNANOECOTOX-Impacts of metal nanoparticles to aquatic ecosystems: from community responses to cellular targets
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s10646-018-1903-ypt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAnimalspt_PT
dc.subjectCadmium compoundspt_PT
dc.subjectGillspt_PT
dc.subjectMetallothioneinpt_PT
dc.subjectMytiluspt_PT
dc.subjectOxidative stresspt_PT
dc.subjectProtein Isoformspt_PT
dc.subjectQuantum dotspt_PT
dc.subjectTelluriumpt_PT
dc.subjectTranscriptionpt_PT
dc.subjectWater Pollutantspt_PT
dc.subjectNanoparticlespt_PT
dc.subjectGene transcriptionpt_PT
dc.subjectEcotoxicitypt_PT
dc.subjectBiomarkerspt_PT
dc.titleChanges in metallothionein transcription levels in the mussel Mytilus galloprovincialis exposed to CdTe quantum dotspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNANOECOTOX-Impacts of metal nanoparticles to aquatic ecosystems: from community responses to cellular targets
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/PTDC%2FAAC-AMB%2F121650%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMAR%2F00350%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Concurso de Projectos de Investigação Científica e Desenvolvimento Tecnológico em todos os Domínios Científicos - 2010/PTDC%2FAAC-AMB%2F121650%2F2010/PT
oaire.citation.endPage410pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage402pt_PT
oaire.citation.titleEcotoxicologypt_PT
oaire.citation.volume27pt_PT
oaire.fundingStreamCOMPETE
oaire.fundingStream5876
oaire.fundingStreamConcurso de Projectos de Investigação Científica e Desenvolvimento Tecnológico em todos os Domínios Científicos - 2010
person.familyNameLopes Rocha
person.familyNameCardoso
person.familyNameBebianno
person.givenNameThiago
person.givenNameCátia
person.givenNameMaria
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0003-0551-6842
person.identifier.orcid0000-0003-3157-9898
person.identifier.orcid0000-0003-1492-8566
person.identifier.ridA-3864-2015
person.identifier.ridI-9235-2014
person.identifier.scopus-author-id37007842200
person.identifier.scopus-author-id7004152715
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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