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Locust bean gum (LBG) – a potential excipient for inhalation purposes: excipient characterisation and in vitro and in vivo toxicological evaluation

2025-09Journal article Open access
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dc.contributor.authorPontes, Jorge Filipe
dc.contributor.authorGuerreiro, Filipa
dc.contributor.authorda Silva, Joana Pinto
dc.contributor.authorAlmeida, Maria
dc.contributor.authorRosso, Annalisa
dc.contributor.authorRosa da Costa, Ana M
dc.contributor.authorAgusti, Géraldine
dc.contributor.authorLollo, Giovanna
dc.contributor.authorGaspar, Maria Manuela
dc.contributor.authorGrenha, Ana
dc.date.accessioned2025-06-27T10:41:54Z
dc.date.available2025-06-27T10:41:54Z
dc.date.issued2025-09
dc.description.abstractDry powders proposed for lung drug delivery typically involve excipients not approved for inhalation. The physicochemical characterisation of excipients is informative regarding their interaction with different body structures. Locust Bean Gum (LBG) has been proposed for diverse applications, including inhalation, benefiting from targeting ability towards phagocytic cells owing to the presence of mannose moieties. The aim of the present study was to characterise the physicochemical parameters of LBG and, for the first time, draw a toxicological profile for this excipient. LBG from three different suppliers was evaluated and eventual changes on polymer characteristics induced by purification and microparticle production were assessed, which were observed to not occur. The commercial samples showed identical FTIR spectra, as well as TGA and DSC profiles, Mw around 3.60 × 106 Da, and Tg near − 39 ◦C. All microparticles presented similar morphology and Feret diameters around 4 μm. In vitro assays performed on respiratory (A549) cells evidenced no impact of LBG microparticles on cell viability (> 80 %) when biorelevant concentrations (≈ 0.1 mg/mL) were used. Additionally, inhalation of LBG microparticles by mice provided indications of a safe profile, without induction of allergic reactions. The basis is laid for further exploration of this material in inhalation.eng
dc.identifier.doi10.1016/j.carbpol.2025.123729
dc.identifier.issn0144-8617
dc.identifier.urihttp://hdl.handle.net/10400.1/27319
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.relationStudy of the impact of dry powder insufflation over respiratory epithelial cells
dc.relationStudy of the impact of dry powder insufflation over respiratory epithelial cells
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relation.ispartofCarbohydrate Polymers
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectDry powder
dc.subjectInhalation
dc.subjectLocust bean gum
dc.subjectLung drug delivery
dc.subjectPolysaccharide
dc.subjectToxicological profile
dc.titleLocust bean gum (LBG) – a potential excipient for inhalation purposes: excipient characterisation and in vitro and in vivo toxicological evaluationeng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleStudy of the impact of dry powder insufflation over respiratory epithelial cells
oaire.awardTitleStudy of the impact of dry powder insufflation over respiratory epithelial cells
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/PD%2FBD%2F137064%2F2018/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//COVID%2FBD%2F152626%2F2022/PT
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oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.citation.startPage123729
oaire.citation.titleCarbohydrate Polymers
oaire.citation.volume363
oaire.fundingStreamOE
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNamePontes
person.familyNameGuerreiro
person.familyNameda Silva
person.familyNameRosa da Costa
person.familyNameGrenha
person.givenNameJorge Filipe
person.givenNameFilipa
person.givenNameJoana Pinto
person.givenNameAna M
person.givenNameAna
person.identifier305029
person.identifier.ciencia-idCB16-5CBD-8B89
person.identifier.ciencia-idBF1D-7EFA-19BC
person.identifier.ciencia-id9318-BAE4-B025
person.identifier.ciencia-idA418-A85E-5DB1
person.identifier.ciencia-id091C-0D58-7225
person.identifier.orcid0000-0002-6447-0242
person.identifier.orcid0000-0001-6224-1553
person.identifier.orcid0000-0002-7706-1544
person.identifier.orcid0000-0003-0225-9537
person.identifier.orcid0000-0002-2136-1396
person.identifier.ridE-2165-2012
person.identifier.ridH-1392-2017
person.identifier.scopus-author-id57195033461
person.identifier.scopus-author-id53986075100
person.identifier.scopus-author-id8607930100
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
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