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Publication
Association of milk traits with SSCP polymorphisms at the growth hormone gene in the Serrana goat
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Advisor(s)
Abstract(s)
The present study suggests the existence of an association between milk production traits and genetic polymorphisms at the
growth hormone (GH) gene in the Portuguese indigenous Serrana goat. TheDNAfrom 229 animals of two ecotypes (Jarmelista
and Ribatejano)was analysed by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique
revealing a high degree of genetic polymorphism at the goat GH (gGH) gene. Two conformation patterns were detected in
exons 1 and 2, 6 in exon 3, 10 in exon 4 and 5 in exon 5. The evaluation of an association effect between these SSCP patterns with milk, fat and protein yields and fat and protein percentages suggests a positive effect of pattern A/B of exon 4 for
Ribatejano ecotype and of pattern A/B of exon 2 for Jarmelista ecotype with milk yield (P < 0.05) and of pattern A/B of
exon 1 and pattern B/B of exon 2 with protein percentage (P < 0.05) for Ribatejano ecotype. The results support the hints
suggested by previous studies of the importance of the gGH gene as a candidate gene for marker-assisted selection in goat
breeds and suggest that exon 4 is a preferential target for further investigation on mutations that influence milk yield variation.
Description
Keywords
Single strand conformation polymorphism Genetic markers Growth hormone Goat Milk traits
Citation
Marques, P. X.; Pereira, M.; Marques, M. Rosário; Santos, Ingrid; Belo, Carmona; Renaville, Robert; Cravador, Alfredo. Association of milk traits with SSCP polymorphisms at the growth hormone gene in the Serrana goat. Small Ruminant Research, 50, 1-2, 177-185, 2003.