Repository logo
 
Publication

Tryptophan modulatory role in European Seabass (Dicentrarchus labrax) immune response to acute inflammation under stressful conditions

2022-10-18Journal article Open access
Simple item page
dc.contributor.authorMachado, Marina
dc.contributor.authorPeixoto, Diogo
dc.contributor.authorSantos, Paulo
dc.contributor.authorRicardo, Ana
dc.contributor.authorDuarte, Inês
dc.contributor.authorCarvalho, Inês
dc.contributor.authorRaquel Cêa de Aragão Teixeira, Cláudia
dc.contributor.authorAzeredo, Rita
dc.contributor.authorCostas, Benjamín
dc.date.accessioned2022-10-28T12:24:36Z
dc.date.available2022-10-28T12:24:36Z
dc.date.issued2022-10-18
dc.date.updated2022-10-26T11:09:00Z
dc.description.abstractThe present work aimed to study the role of dietary tryptophan supplementation in modulating the European seabass (Dicentrarchus labrax) immune condition during stressful rearing conditions (i.e., 15 days exposure to high density), as well as the immune response to acute inflammation after intraperitoneal injection of a bacterial pathogen. Stress alone did not compromise seabass health indicators. In contrast, a clear peripheral and local inflammatory response was observed in response to the inoculated bacteria. Moreover, exposure to a high stocking density seemed to exacerbate the inflammatory response at early sampling points, compared to fish stocked at a lower density. In contrast, stressed fish presented some immune-suppressing effects on the T-cell surface glycoprotein receptor expressions at a late sampling point following inflammation. Regarding the effects of dietary tryptophan, no changes were observed on seabass immune indicators prior to inflammation, while a small number of immunosuppressive effects were observed in response to inflammation, supporting tryptophan’s role in the promotion of immune-tolerance signals during inflammation. Nonetheless, tryptophan dietary supplementation improved the inflammatory response against a bacterial pathogen during stressful conditions, supported by a reduction of plasma cortisol levels, an up-regulation of several immune-related genes at 48 h, and an inversion of the previously observed, stress-induced T-cell suppression. Finally, the involvement of tryptophan catabolism in macrophages was confirmed by the up-regulation of genes involved in the kynurenine pathway. The present study brings new insights regarding the immune modulatory role of tryptophan during stressful conditions in fish, thus allowing for the development of novel prophylactic protocols during vaccination by intraperitoneal injection in the European seabass.pt_PT
dc.description.sponsorshipORBEA-CIIMAR_26_2018; 0421/000/000/2020
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationInternational Journal of Molecular Sciences 23 (20): 12475 (2022)pt_PT
dc.identifier.doi10.3390/ijms232012475pt_PT
dc.identifier.eissn1422-0067
dc.identifier.urihttp://hdl.handle.net/10400.1/18458
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationInterdisciplinary Centre of Marine and Environmental Research
dc.relationInterdisciplinary Centre of Marine and Environmental Research
dc.relationThe modulatory role of tryptophan on the neuroendocrine and immune crosstalk
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.titleTryptophan modulatory role in European Seabass (Dicentrarchus labrax) immune response to acute inflammation under stressful conditionspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleInterdisciplinary Centre of Marine and Environmental Research
oaire.awardTitleInterdisciplinary Centre of Marine and Environmental Research
oaire.awardTitleThe modulatory role of tryptophan on the neuroendocrine and immune crosstalk
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FCVT-CVT%2F32349%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCVT-CVT%2F7741%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04423%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04423%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//UI%2FBD%2F150900%2F2021/PT
oaire.citation.issue20pt_PT
oaire.citation.startPage12475pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume23pt_PT
oaire.fundingStream9471 - RIDTI
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameAragão
person.givenNameCláudia
person.identifier174173
person.identifier.ciencia-idD01E-0726-9F3E
person.identifier.orcid0000-0002-9217-0183
person.identifier.ridK-2310-2012
person.identifier.scopus-author-id55903315700
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationbc649967-3b39-41dc-8fab-b60c24e65286
relation.isAuthorOfPublication.latestForDiscoverybc649967-3b39-41dc-8fab-b60c24e65286
relation.isProjectOfPublicationf871bc9b-6a80-48a4-9bac-102fb4f60901
relation.isProjectOfPublication0643f764-d6af-4fb0-8049-9e4c91e4ddea
relation.isProjectOfPublication2918f082-1ced-47c5-a6ea-eff9b8eaca1f
relation.isProjectOfPublicationb2e68640-2978-4601-a400-c2eb842438da
relation.isProjectOfPublication968dc975-04f8-4b66-a9dc-7a3958c0eb93
relation.isProjectOfPublication.latestForDiscovery0643f764-d6af-4fb0-8049-9e4c91e4ddea

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
ijms-23-12475.pdf
Size:
894.81 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.46 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)