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Highly polymorphic microsatellite loci for the Parsley frog (Pelodytes punctatus): characterization and testing for cross-species amplification
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Orientador(es)
Resumo(s)
A microsatellite library was developed using
genomic DNA of the Parsley frog, Pelodytes punctatus, an amphibian species which inhabits Mediterranean temporary pond systems. Number of alleles and heterozygosity ranged from 10 to 25 and from 0.66 to 0.90, respectively. Crossspecies
amplification was successful for 13 of the 15 developed loci for the related species, Pelodytes ibericus. The high levels of polymorphism revealed by these loci will be
extremely useful for characterizing the population genetic diversity and structure and to estimate levels of dispersal and
gene flow in the species P. punctatus and P. ibericus.
Descrição
Palavras-chave
Pelodytes punctatus Parsley frog Microsatellites Cross-species amplification Temporary ponds Amphibian conservation genetics
Contexto Educativo
Citação
Van De Vliet, M.S.; Diekmann, O.E.; Serrão, E.T.A.; Beja, P. Highly polymorphic microsatellite loci for the Parsley frog (Pelodytes punctatus): Characterization and testing for cross-species amplification, Conservation Genetics, 10, 3, 665-668, 2009.
Editora
Springer