A microsatellite sequence in the fifth intron provides a broad-spectrum SSR marker for multiple alleles of the er1/PsMLO1 powdery mildew resistance gene in Pisum sativum L.

Cardoso, André; Pereira, Ricardo; Fonseca, Maria; M Leitão, J

http://hdl.handle.net/10400.1/13072

Use this identifier to reference this record.

Name:	Description:	Size:	Format:
cardoso2017.pdf		1 MB	Adobe PDF	Download

Send Feedback

Authors

Abstract(s)

Powdery mildew caused by the biotrophic ascomycete fungus Erysiphe pisi Syd. is one the most devastating diseases of peas (Pisum sativum L.) with enormous impact in seed production. The most efficient genetic resistance to this disease, so far identified, is conferred by the naturally occurring or experimentally induced by chemical mutagenesis recessive state of the locus er1. Genetically mapped over 2 decades ago, this gene was recently identified as a homolog of the barley (Hordeum sativum L.) powdery mildew resistance gene MLO, and renamed as PsMLO1. The broad wide resistance conferred by the er1/PsMLO1 locus was found to be a consequence of the loss of function of the encoded PsMLO1 protein. After the publication of the expressed sequence of this gene by another research group, we published the genomic sequences of this gene which harbors a relatively long (TA) microsatellite sequence (SSR) in the fifth intron. SSR markers based on this highly polymorphic microsatellite can be used for marker-assisted selection in multiple pea powdery mildew resistance breeding programs involving the er1/PsMLO1 resistance, except in the rare circumstances where the progenitor lines are monomorphic for the microsatellite sequence.