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- A microsatellite sequence in the fifth intron provides a broad-spectrum SSR marker for multiple alleles of the er1/PsMLO1 powdery mildew resistance gene in Pisum sativum L.Publication . Cardoso, André; Pereira, Ricardo; Fonseca, Maria; M Leitão, JPowdery mildew caused by the biotrophic ascomycete fungus Erysiphe pisi Syd. is one the most devastating diseases of peas (Pisum sativum L.) with enormous impact in seed production. The most efficient genetic resistance to this disease, so far identified, is conferred by the naturally occurring or experimentally induced by chemical mutagenesis recessive state of the locus er1. Genetically mapped over 2 decades ago, this gene was recently identified as a homolog of the barley (Hordeum sativum L.) powdery mildew resistance gene MLO, and renamed as PsMLO1. The broad wide resistance conferred by the er1/PsMLO1 locus was found to be a consequence of the loss of function of the encoded PsMLO1 protein. After the publication of the expressed sequence of this gene by another research group, we published the genomic sequences of this gene which harbors a relatively long (TA) microsatellite sequence (SSR) in the fifth intron. SSR markers based on this highly polymorphic microsatellite can be used for marker-assisted selection in multiple pea powdery mildew resistance breeding programs involving the er1/PsMLO1 resistance, except in the rare circumstances where the progenitor lines are monomorphic for the microsatellite sequence.
- Establishment of a broad-spectrum marker for er1/PsMLO1 powdery mildew resistance in pea (Pisum sativum L.)Publication . Cardoso, André Filipe Dias Almeida; Leitão, J. M.O oídio, causado pelo fungo ascomycete Erysiphe pisi Syd, é uma das doenças mais importantes que afectam a produção de ervilha (Pisum sativum L.) em Portugal e em países por todo o mundo (Sousa, 1999). Esta doença prospera em climas húmidos temperados causando perdas até 50% nas colheitas de ervilha, uma leguminosa muito importante e vastamente cultivada na Europa, sendo a quarta mais cultivada em todo o mundo (Rubiales et al., 2009; Warkentin et al., 1996).
- Tagging mortality in acoustic telemetry studies: insights from a multispecies analysisPublication . Martínez Ramírez, Lucas; Bentes, Luis; Dias Almeida Cardoso, André Filipe; Erzini, Karim; Gandra, Miguel; Kraft, Sebastian; Winkler, Alexander; Abecasis, DavidThe widespread adoption of acoustic telemetry has transformed our understanding of marine species' behavior and movement ecology. However, accurately interpreting telemetry data, especially concerning tagging mortality, is essential for drawing valid conclusions. In this study, we scrutinized tagging mortality in 223 individuals across 14 species and evaluated the impact of tagging methodologies, including capture method and size effects. Results reveal high tagging survival rates overall, attributable to the resiliency of the studied species and the rigorous animal welfare protocols followed during tagging procedures. Our results highlight the importance of tailoring array designs to the specific mobility patterns of the studied species for accurate survival assessments. This research contributes to generalizing mortality assessments and clearing the path for more precise and reliable telemetry studies in the future.