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An efficient procedure to extract RNA from a single Aphis gossypii and Aphis spiraecola for detecting Citrus tristeza virus by nested RT-PCR

2022Journal article Open access
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dc.contributor.authorDandlen, Susana Anahi
dc.contributor.authorRusso, Guilherme
dc.contributor.authorHorta, Soraia
dc.contributor.authorDuarte, Amilcar
dc.contributor.authorPaiva, Paulo
dc.contributor.authorTomás Marques, Natália
dc.date.accessioned2025-05-05T12:59:07Z
dc.date.available2025-05-05T12:59:07Z
dc.date.issued2022en_US
dc.date.updated2025-04-27T09:19:33Z
dc.description.abstractA simple protocol to extract RNA from a single wingless aphid, Aphis gossypii and Aphis spiraecola, using liquid nitrogen and Tri-Reagent is described. A nested reverse-transcription polymerase chain reaction with degenerate primers enabled Citrus tristeza virus (CTV) detection in these aphid species collected from an infected tangor ‘Ortanique’ (Citrus × sinensis (L.) Osbeck × Citrus reticulata Blanco) in the field. The procedure enabled a CTV detection rate of 75% in the A. gossypii and A. spiraecola evaluated.eng
dc.description.sponsorshipPTDC/BAA-AGR/30957/2027
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.slugcv-prod-4480455
dc.identifier.urihttp://hdl.handle.net/10400.1/27083
dc.language.isoeng
dc.peerreviewedyes
dc.publisherResearch Trends
dc.relationCenter for Electronics, Optoelectronics and Telecommunications
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAphid
dc.subjectCTV
dc.subjectDetection
dc.subjectDiagnostic
dc.subjectRNA extraction
dc.titleAn efficient procedure to extract RNA from a single Aphis gossypii and Aphis spiraecola for detecting Citrus tristeza virus by nested RT-PCReng
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCenter for Electronics, Optoelectronics and Telecommunications
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00631%2F2020/PT
oaire.citation.endPage22
oaire.citation.startPage15
oaire.citation.titleCurrent Topics in Virologyen_US
oaire.citation.volume19en_US
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameDandlen
person.familyNameDuarte
person.familyNameTomás Marques
person.givenNameSusana Anahi
person.givenNameAmilcar
person.givenNameNatália
person.identifier.ciencia-id6613-CD28-84BE
person.identifier.ciencia-id081B-5FD8-0E51
person.identifier.ciencia-id1510-CD2F-81CE
person.identifier.orcid0000-0002-2763-1916
person.identifier.orcid0000-0002-0222-621X
person.identifier.ridF-1472-2010
person.identifier.scopus-author-id7102602102
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.cv.cienciaid081B-5FD8-0E51 | Amílcar Manuel Marreiros Duarte
rcaap.rightsopenAccessen_US
relation.isAuthorOfPublication76f4b029-5f3f-4d05-b8a5-597c2e6a91e8
relation.isAuthorOfPublication88280e66-023a-49f6-8081-c827a15900ce
relation.isAuthorOfPublication4b1751aa-958b-4d68-ae97-ea628673ba00
relation.isAuthorOfPublication.latestForDiscovery76f4b029-5f3f-4d05-b8a5-597c2e6a91e8
relation.isProjectOfPublication77b70459-1e8c-4a6c-9856-58860aaddb6b
relation.isProjectOfPublication.latestForDiscovery77b70459-1e8c-4a6c-9856-58860aaddb6b

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