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Two estrogen receptors expressed in the teleost fish, Sparus aurata: cDNA cloning, characterization and tissue distribution

2000Journal article Restricted access
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dc.contributor.authorSocorro, Sílvia
dc.contributor.authorPower, Deborah
dc.contributor.authorOlsson, P. E.
dc.contributor.authorCanario, Adelino V. M.
dc.date.accessioned2014-05-09T16:44:04Z
dc.date.available2014-05-09T16:44:04Z
dc.date.issued2000
dc.date.updated2014-05-09T10:25:25Z
dc.description.abstractEstrogen is an essential hormone for many reproductive and non-reproductive functions. The function of estrogen in the reproductive cycle of seabream (Sparus aurata), a protandrous hermaphrodite teleost fish, is complex but it is understood to be involved in sex inversion, a process that occurs in some individuals during the second reproductive season. Estrogen action is mediated by two estrogen receptor (ER) subtypes designated alpha and beta. As a step to understanding the mechanisms of estrogen action during natural and induced sex reversal in seabream, we have isolated two cDNAs encoding distinct forms of ER homologous to mammalian ERalpha and ERbeta. The seabream ERalpha clone (sbERalpha1), which was truncated in the A/B domain, corresponded to a variant differing in five amino acids from another recently cloned sbERalpha. The ERbeta clone (sbERbeta) encoded a protein 559 amino acids long and showed only 40% identity to sbERalpha. Northern blot analysis of liver and ovary mRNA indicated the presence of several transcripts of the two receptor subtypes. PCR analysis showed that the two receptors differed in their expression pattern. sbERalpha had a more restricted distribution, occurring mainly in testis, liver and heart, and sbERbeta was present in most tissues, being more abundant in ovary, testis, liver, intestine and kidney. The presence in seabream of two ERs with several ER transcripts and their pattern of distribution are consistent with the widespread effects of estrogen in different tissues.por
dc.identifier.citationSocorro, S.; Power, D. M.; Olsson, P. E.; Canario, A. V. M.Two estrogen receptors expressed in the teleost fish, Sparus aurata: cDNA cloning, characterization and tissue distribution, Journal of Endocrinology, 166, 2, 293-306, 2000por
dc.identifier.otherAUT: DPO00386; ACA00258;
dc.identifier.urihttp://hdl.handle.net/10400.1/3841
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSociety for Endocrinologypor
dc.titleTwo estrogen receptors expressed in the teleost fish, Sparus aurata: cDNA cloning, characterization and tissue distributionpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage306por
oaire.citation.startPage293por
oaire.citation.titleJournal of Endocrinologypor
oaire.citation.volume166por
person.familyNameSocorro
person.familyNamePower
person.familyNameCanario
person.givenNameSílvia Cristina da Cruz Marques
person.givenNameDeborah Mary
person.givenNameAdelino
person.identifierhttps://scholar.google.pt/citations?user=PA3L_h8AAAAJ&hl=pt-PT
person.identifier143624
person.identifier.ciencia-idAB17-5C25-1F2C
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0001-8181-488X
person.identifier.orcid0000-0003-1366-0246
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id23097994600
person.identifier.scopus-author-id7101806760
person.identifier.scopus-author-id56568523700
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationa45b720d-7f58-452d-a4bf-e9a78a9aab6e
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0

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