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Exploring Caralluma europaea (Guss.) NEBr. as a potential source of bioactive molecules: In vitro antioxidant and antidiabetic properties, and phenolic profile of crude extracts and fractions

2019-11Journal article Restricted
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dc.contributor.authorDra, Loubna Ait
dc.contributor.authorRodrigues, Maria Joao
dc.contributor.authorNeng, Nuno da Rosa
dc.contributor.authorNogueira, Jose M. F.
dc.contributor.authorElamine, Youssef
dc.contributor.authorAghraz, Abdellah
dc.contributor.authorMarkouk, Mohamed
dc.contributor.authorLarhsini, Mustapha
dc.contributor.authorCustódio, Luísa
dc.date.accessioned2020-07-24T10:51:12Z
dc.date.available2020-07-24T10:51:12Z
dc.date.issued2019-11
dc.description.abstractCaralluma europaea (Guss.) N.E.Br is a succulent medicinal species used in the Moroccan traditional medicine for its anticancer and antidiabetic properties. In this work, this species was explored for the in vitro antidiabetic and antioxidant properties of crude methanol and dichloromethane extracts, and obtained fractions, from its aerial organs. In vitro antidiabetic properties were evaluated towards baker's yeast and rat's intestinal alpha-glucosidase, and alpha-amylase. Four in vitro methods were used to appraise the antioxidant potential, namely radical scavenging activity (RSA) on 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS), and iron and copper chelating assays. Finally, the profile of the main phenolic compounds present in the samples was assessed by high-performance liquid chromatography equipped with diodearray detection (HPLC-DAD). The highest inhibition of baker's yeast alpha-glucosidase was obtained with the methanol extract (87%), and its fractions, with a maximum inhibition of 83% at the concentration of 10 mg/ml. The most significant inhibition of mammalian alpha-glucosidase was obtained with the dichloromethane crude extract (85%), and corresponding fractions at 10 mg/ml. Moderate activity was achieved with the two extracts against a-amylase (52-53%) at 10 mg/ml, but no relevant activity was detected for most fractions. Crude extracts had a significant RSA against DPPH and ABTS, with range values of 83-98% (methanol extracts) and 53-80% (dichloromethane extracts). Fractions from the methanol extract were more active against tested radicals. Crude extracts had chelating activity on both metals, and the highest copper chelation was achieved with the methanol extract, while the dichloromethane extract and the majority of fractions from both extracts presented the highest iron chelation at 10 mg/ml. A total of 21 phenolic compounds were identified and quantified by HPLC-DAD in the samples, and the main compounds detected were catechine hydrate and salicylic and caffeic acids. Our results suggest that C. europaea extracts contain compounds with in vitro antioxidant and antidiabetic properties with potential applications in the pharmaceutical and food industries.
dc.description.sponsorshipFoundation for Science and Technology (FCT)Portuguese Foundation for Science and Technology
dc.description.sponsorshipPortuguese National Budget [CCMAR/Multi/04326]
dc.description.sponsorshipFCT Scientific Employment Stimulus [CEECIND/00425/2017]
dc.description.sponsorshipFCTPortuguese Foundation for Science and Technology [SFRH/BD/116604/2016]
dc.description.sponsorshipFCT Investigator Programme [IF/00049/2012]
dc.identifier.doi10.1016/j.indcrop.2019.111527
dc.identifier.issn0926-6690
dc.identifier.issn1872-633X
dc.identifier.urihttp://hdl.handle.net/10400.1/14204
dc.language.isoeng
dc.peerreviewedyes
dc.publisherElsevier
dc.subjectKey enzymes relevant
dc.subjectAlpha-glucosidase
dc.subjectPolyphenolic compounds
dc.subjectInhibitory-activity
dc.subjectOxidative stress
dc.subjectMedicinal-plants
dc.subjectAcid
dc.subjectAssociation
dc.subjectMechanism
dc.subjectLuteolin
dc.titleExploring Caralluma europaea (Guss.) NEBr. as a potential source of bioactive molecules: In vitro antioxidant and antidiabetic properties, and phenolic profile of crude extracts and fractions
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04326%2F2013/PT
oaire.citation.startPageUNSP 111527
oaire.citation.titleIndustrial Crops and Products
oaire.citation.volume139
oaire.fundingStream5876
person.familyNameRodrigues
person.familyNameCustódio
person.givenNameMaria João
person.givenNameLuísa
person.identifierR-004-VNG
person.identifier.ciencia-id2514-0E17-1D8D
person.identifier.ciencia-id791B-C560-AEA2
person.identifier.orcid0000-0001-8732-710X
person.identifier.orcid0000-0003-4338-7703
person.identifier.ridM-6101-2013
person.identifier.scopus-author-id56031608100
person.identifier.scopus-author-id15831018900
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
rcaap.typearticle
relation.isAuthorOfPublication12c32925-de9e-4289-bdd5-1d655d7a278c
relation.isAuthorOfPublicationf9cfed0f-6b67-413e-988c-ac7397183471
relation.isAuthorOfPublication.latestForDiscovery12c32925-de9e-4289-bdd5-1d655d7a278c
relation.isProjectOfPublication868b4818-3efa-4edb-9202-c464d64fd38f
relation.isProjectOfPublication.latestForDiscovery868b4818-3efa-4edb-9202-c464d64fd38f

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