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PTHrP regulation and calcium balance in sea bream (Sparus auratus L.) under calcium constraint

2006Journal article Open access
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dc.contributor.authorAbbink, W.
dc.contributor.authorBevelander, G. S.
dc.contributor.authorHang, X. M.
dc.contributor.authorLu, W. Q.
dc.contributor.authorGuerreiro, P. M.
dc.contributor.authorSpanings, T.
dc.contributor.authorCanario, Adelino V. M.
dc.contributor.authorFlik, G.
dc.date.accessioned2014-05-20T11:54:31Z
dc.date.available2014-05-20T11:54:31Z
dc.date.issued2006
dc.date.updated2014-05-13T09:37:15Z
dc.description.abstractJuvenile gilthead sea bream were exposed to diluted seawater (2.5‰ salinity; DSW) for 3·h or, in a second experiment, acclimated to DSW and fed a control or calcium-deficient diet for 30·days. Branchial Ca2+ influx, drinking rate and plasma calcium levels were assessed. Sea bream plasma parathyroid hormone related protein (sPTHrP) was measured, and mRNAs of pthrp, its main receptor, pth1r, and the calcium-sensing receptor (casr) were quantified in osmoregulatory tissues and the pituitary gland. When calcium is limited in water or diet, sea bream maintain calcium balance; however, both plasma Ca2+ and plasma sPTHrP concentrations were lower when calcium was restricted in both water and diet. Positive correlations between plasma sPTHrP and plasma Ca2+ (R2=0.30, N=39, P<0.05), and plasma sPTHrP and body mass of the fish (R2=0.37, N=148, P<0.001) were found. Immunoreactive sPTHrP was demonstrated in pituitary gland pars intermedia cells that border the pars nervosa and co-localises with somatolactin. In the pituitary gland, pthrp, pth1r and casr mRNAs were downregulated after both short- and long-term exposure to DSW. A correlation between pituitary gland pthrp mRNA expression and plasma Ca2+ (R2=0.71, N=7, P<0.01) was observed. In gill tissue, pthrp and pth1r mRNAs were significantly upregulated after 30·days exposure to DSW, whereas no effect was found for casr mRNA expression. We conclude that in water of low salinity, declining pituitary gland pthrp mRNA expression accompanied by constant plasma sPTHrP levels points to a reduced sPTHrP turnover and that sPTHrP, through paracrine interaction, is involved in the regulation of branchial calcium handling, independently of endocrine pituitary gland sPTHrP.por
dc.identifier.citationAbbink, W.; Bevelander, G. S.; Hang, X. M.; Lu, W. Q.; Guerreiro, P. M.; Spanings, T.; Canario, A. V. M.; Flik, G. PTHrP regulation and calcium balance in sea bream (Sparus auratus L.) under calcium constraint, Journal of Experimental Biology, 209, 18, 3550-3557, 2006.por
dc.identifier.doihttp:// dx.doi.org/10.1242/jeb.02399
dc.identifier.issn0022-0949
dc.identifier.otherAUT: ACA00258;
dc.identifier.urihttp://hdl.handle.net/10400.1/4006
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherThe Company of Biologistspor
dc.subjectSparus auratuspor
dc.subjectHypocalcemiapor
dc.subjectPTHrPpor
dc.subjectCalcium balancepor
dc.subjectPituitary glandpor
dc.titlePTHrP regulation and calcium balance in sea bream (Sparus auratus L.) under calcium constraintpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage3557por
oaire.citation.issue18por
oaire.citation.startPage3550por
oaire.citation.titleJournal of Experimental Biologypor
oaire.citation.volume209por
person.familyNameGuerreiro
person.familyNameCanario
person.givenNamePedro Miguel
person.givenNameAdelino
person.identifierA-2539-2009
person.identifier143624
person.identifier.ciencia-id5C13-965D-3148
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.orcid0000-0001-5371-7919
person.identifier.orcid0000-0002-6244-6468
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id56568523700
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationd0a8a148-98e7-4899-8fb0-0b8afa91e4a4
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublication.latestForDiscoveryd0a8a148-98e7-4899-8fb0-0b8afa91e4a4

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