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Olfactory sensitivity to steroid glucuronates in Mozambique tilapia suggests two distinct and specific receptors for pheromone detection

2014-12Journal article Open Access
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dc.contributor.authorKeller-Costa, Tina
dc.contributor.authorCanario, Adelino V. M.
dc.contributor.authorHubbard, Peter
dc.date.accessioned2018-12-07T14:52:48Z
dc.date.available2018-12-07T14:52:48Z
dc.date.issued2014-12
dc.description.abstractCichlids offer an exciting opportunity to understand vertebrate speciation; chemical communication could be one of the drivers of African cichlid radiation. Chemical signals mediate key aspects in the lives of vertebrates and often are species specific. Dominant male Mozambique tilapia [Oreochromis mossambicus (Peters 1852)] release a sex pheromone, 5 beta-pregnan-3 alpha,17 alpha,20 beta-triol 3-glucuronate and its 20 alpha-epimer, via their urine. The objective of this study was to assess the sensitivity, specificity and versatility of the olfactory system of O. mossambicus to other steroids and their conjugates using the electro-olfactogram. Oreochromis mossambicus was sensitive to several 3-glucuronidated steroids, but did not respond to prostaglandins, unconjugated steroids or 17- or 20-conjugated steroids. Stimulation of the olfactory epithelium with increasing concentrations (1 pmol l(-1) to 10 mu mol l(-1)) of 5 beta-pregnan-3 alpha, 17 alpha,20 beta-triol 3-glucuronate, 5 beta-pregnan-3 alpha, 17 alpha,20 beta-triol 3-glucuronate, 3 alpha,17 alpha-dihydroxy-5 beta-pregnan-20-one 3-glucuronate, etiocholanolone 3 alpha-glucuronate and 17 beta-estradiol 3-glucuronate produced characteristic sigmoidal concentration-response curves. However, tilapia were most sensitive to 17 beta-estradiol-3-glucuronate, which also had the lowest apparent EC50 and maximal response amplitude. Cross-adaptation and binary mixture experiments suggested that 5 beta,3 alpha-reduced pregnan-and androstan-3-glucuronates share (a) common olfactory receptor(s), whereas 17 beta-estradiol 3-glucuronate is detected via (a) distinct olfactory receptor(s). In conclusion, the Mozambique tilapia has evolved high olfactory sensitivity and specificity to 3-glucuronidated steroids through two distinct olfactory receptor types; one detecting a male sex pheromone and a second detecting 17 beta-estradiol 3-glucuronate, a putative female-derived signal. However, O. mossambicus differs markedly in its olfactory perception from the more recently derived East African cichlid Astatotilapia burtoni, suggesting that chemical communication could, indeed, be involved in speciation.
dc.description.sponsorshipScience and Technology Foundation (FCT), Portugal [POCI/BIA-BDE/55463/2004]; FCT [SFRH/BD/46192/2008]
dc.description.versioninfo:eu-repo/semantics/publishedVersion
dc.identifier.doi10.1242/jeb.111518
dc.identifier.issn0022-0949
dc.identifier.issn1477-9145
dc.identifier.urihttp://hdl.handle.net/10400.1/11218
dc.language.isoeng
dc.peerreviewedyes
dc.publisherCompany of Biologists
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectGobies Neogobius-Melanostomus
dc.subjectAfrican cichlid fish
dc.subjectOreochromis-Mossambicus
dc.subjectProstaglandins function
dc.subjectHaplochromis-Burtoni
dc.subjectBehavioral-responses
dc.subjectIctalurus-punctatus
dc.subjectElectro-olfactogram
dc.subjectF-Prostaglandins
dc.subjectChannel catfish
dc.titleOlfactory sensitivity to steroid glucuronates in Mozambique tilapia suggests two distinct and specific receptors for pheromone detection
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POCI/POCI%2FBIA-BDE%2F55463%2F2004/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F46192%2F2008/PT
oaire.citation.endPage4212
oaire.citation.issue23
oaire.citation.startPage4203
oaire.citation.titleJournal of Experimental Biology
oaire.citation.volume217
oaire.fundingStreamPOCI
oaire.fundingStreamSFRH
person.familyNameKeller-Costa
person.familyNameCanario
person.familyNameHubbard
person.givenNameTina
person.givenNameAdelino
person.givenNamePeter
person.identifier143624
person.identifier.ciencia-id9319-A7D5-5335
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.ciencia-id951F-BA4B-A21F
person.identifier.orcid0000-0003-3702-9192
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0002-3007-4647
person.identifier.ridC-7942-2009
person.identifier.ridB-2823-2008
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id7005928467
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
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