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Characterization and comparison of bacterial communities of an invasive and two native Caribbean seagrass species sheds light on the possible influence of the microbiome on invasive mechanisms

2021-08Journal article Open access
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dc.contributor.authorAires, Tania
dc.contributor.authorStuij, Tamara M.
dc.contributor.authorMuyzer, Gerard
dc.contributor.authorSerrao, Ester
dc.contributor.authorEngelen, Aschwin
dc.date.accessioned2021-09-14T09:15:01Z
dc.date.available2021-09-14T09:15:01Z
dc.date.issued2021-08
dc.description.abstractInvasive plants, including marine macrophytes, are one of the most important threats to biodiversity by displacing native species and organisms depending on them. Invasion success is dependent on interactions among living organisms, but their study has been mostly limited to negative interactions while positive interactions are mostly underlooked. Recent studies suggested that microorganisms associated with eukaryotic hosts may play a determinant role in the invasion process. Along with the knowledge of their structure, taxonomic composition, and potential functional profile, understanding how bacterial communities are associated with the invasive species and the threatened natives (species-specific/environmentally shaped/tissue-specific) can give us a holistic insight into the invasion mechanisms. Here, we aimed to compare the bacterial communities associated with leaves and roots of two native Caribbean seagrasses (Halodule wrightii and Thalassia testudinum) with those of the successful invader Halophila stipulacea, in the Caribbean island Curaçao, using 16S rRNA gene amplicon sequencing and functional prediction. Invasive seagrass microbiomes were more diverse and included three times more species-specific core OTUs than the natives. Associated bacterial communities were seagrass-specific, with higher similarities between natives than between invasive and native seagrasses for both communities associated with leaves and roots, despite their strong tissue differentiation. However, with a higher number of OTUs in common, the core community (i.e., OTUs occurring in at least 80% of the samples) of the native H. wrightii was more similar to that of the invader H. stipulacea than T. testudinum, which could reflect more similar essential needs (e.g., nutritional, adaptive, and physiological) between native and invasive, in contrast to the two natives that might share more environment-related OTUs. Relative to native seagrass species, the invasive H. stipulacea was enriched in halotolerant bacterial genera with plant growth-promoting properties (like Halomonas sp. and Lysinibacillus sp.) and other potential beneficial effects for hosts (e.g., heavy metal detoxifiers and quorum sensing inhibitors). Predicted functional profiles also revealed some advantageous traits on the invasive species such as detoxification pathways, protection against pathogens, and stress tolerance. Despite the predictive nature of our findings concerning the functional potential of the bacteria, this investigation provides novel and important insights into native vs. invasive seagrasses microbiome. We demonstrated that the bacterial community associated with the invasive seagrass H. stipulacea is different from native seagrasses, including some potentially beneficial bacteria, suggesting the importance of considering the microbiome dynamics as a possible and important influencing factor in the colonization of non-indigenous species. We suggest further comparison of H. stipulacea microbiome from its native range with that from both the Mediterranean and Caribbean habitats where this species has a contrasting invasion success. Also, our new findings open doors to a more in-depth investigation combining meta-omics with bacterial manipulation experiments in order to confirm any functional advantage in the microbiome of this invasive seagrass.pt_PT
dc.description.sponsorshipCEECINST/00114/2018pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3389/fmicb.2021.653998pt_PT
dc.identifier.eissn1664-302X
dc.identifier.urihttp://hdl.handle.net/10400.1/17102
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontiers Mediapt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSeagrassespt_PT
dc.subjectInvasionspt_PT
dc.subjectAdaptationpt_PT
dc.subjectHost–microbe interactionpt_PT
dc.subjectDrivers of microbiomept_PT
dc.subjectMarine holobiontspt_PT
dc.titleCharacterization and comparison of bacterial communities of an invasive and two native Caribbean seagrass species sheds light on the possible influence of the microbiome on invasive mechanismspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/63018/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/77788/PT
oaire.citation.titleFrontiers in Microbiologypt_PT
oaire.citation.volume12pt_PT
oaire.fundingStream6817
oaire.fundingStreamOE
oaire.fundingStreamOE
person.familyNameAires
person.familyNameSerrao
person.familyNameEngelen
person.givenNameTania
person.givenNameEster A.
person.givenNameAschwin
person.identifier282868
person.identifierC-6686-2012
person.identifier.ciencia-id4318-9E2C-32B9
person.identifier.ciencia-id5B13-B26E-B1EC
person.identifier.ciencia-id911A-9A0C-744D
person.identifier.orcid0000-0002-1964-6819
person.identifier.orcid0000-0003-1316-658X
person.identifier.orcid0000-0002-9579-9606
person.identifier.ridM-8306-2013
person.identifier.ridM-3432-2013
person.identifier.scopus-author-id7004093604
person.identifier.scopus-author-id6701622770
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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