Publication
Peroxynitrite induces F-actin depolymerization and blockade of myosin ATPase stimulation
| dc.contributor.author | Tiago, Teresa | |
| dc.contributor.author | Ramos, Susana | |
| dc.contributor.author | Aureliano, M. | |
| dc.contributor.author | Gutiérrez-Merino, Carlos | |
| dc.date.accessioned | 2012-06-26T10:17:50Z | |
| dc.date.available | 2012-06-26T10:17:50Z | |
| dc.date.issued | 2006 | |
| dc.description.abstract | Treatment of F-actin with the peroxynitrite-releasing agent 3-morpholinosydnonimine (SIN-1) produced a dose-dependent F-actin depolymerization. This is due to released peroxynitrite because it is not produced by ‘decomposed SIN-1’, and it is prevented by superoxide dismutase concentrations efficiently preventing peroxynitrite formation. F-actin depolymerization has been found to be very sensitive to peroxynitrite, as exposure to fluxes as low as 50–100 nM peroxynitrite leads to nearly 50% depolymerization in about 1 h. G-actin polymerization is also impaired by peroxynitrite although with nearly 2-fold lower sensitivity. Exposure of F-actin to submicromolar fluxes of peroxynitrite produced cysteine oxidation and also a blockade of the ability of actin to stimulate myosin ATPase activity. Our results suggest that an imbalance of the F-actin/G-actin equilibrium can account for the observed structural and functional impairment of myofibrils under the peroxynitrite-mediated oxidative stress reported for some pathophysiological conditions. | por |
| dc.identifier.issn | 0006-291X | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/1292 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | Elsevier | por |
| dc.subject | Actin | por |
| dc.subject | Peroxynitrite | por |
| dc.title | Peroxynitrite induces F-actin depolymerization and blockade of myosin ATPase stimulation | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F20777%2F2004/PT | |
| oaire.citation.endPage | 49 | por |
| oaire.citation.issue | 342 | por |
| oaire.citation.startPage | 44 | por |
| oaire.citation.title | Biochemical and Biophysical Research Communications | por |
| oaire.fundingStream | SFRH | |
| person.familyName | Aureliano | |
| person.familyName | Gutierrez-Merino | |
| person.givenName | Manuel | |
| person.givenName | Carlos | |
| person.identifier | 584146 | |
| person.identifier.ciencia-id | AA14-3490-DC5E | |
| person.identifier.orcid | 0000-0003-4858-3201 | |
| person.identifier.orcid | 0000-0003-3673-7007 | |
| person.identifier.rid | I-3283-2012 | |
| person.identifier.rid | K-4574-2014 | |
| person.identifier.scopus-author-id | 6603412860 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |
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