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Root ferric chelate reductase is regulated by iron and copper in straberry plants

2013Journal article Restricted access
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dc.contributor.authorPestana, M.
dc.contributor.authorCorreia, P. J.
dc.contributor.authorSaavedra, Teresa
dc.contributor.authorGama, Florinda
dc.contributor.authorDandlen, S.
dc.contributor.authorNolasco, Gustavo
dc.contributor.authorVarennes, Amarilis de
dc.date.accessioned2015-06-15T14:13:45Z
dc.date.available2015-06-15T14:13:45Z
dc.date.issued2013
dc.description.abstractIn the present experiment, we studied the interaction between copper (Cu) and iron (Fe) in strawberry plants grown in nutrient solutions containing different concentrations of Fe. Plants grown in the absence of iron (Fe0) had the characteristic symptoms of Fe deficiency, with smaller chlorotic leaves, less biomass, acidification of the nutrient solution, and roots that were smaller and less ramified, while no symptoms of Fe deficiency were observed in plants grown with Fe. A greater amount of Cu was found in roots of chlorotic plants than in those grown with Fe, while plants grown with 20M of Fe (Fe20) in the nutrient solution had a greater amount of Fe compared with plants from the other treatments. Chlorotic plants (Fe0) and plants grown with the greatest level of Fe (Fe20) had a greater root ferric chelate reductase (FC-R; EC 1.16.1.17) activity compared with the other treatments with 5 or 10M Fe in the nutrient solution. The same pattern was obtained for relative FC-R mRNA concentration and for the sum of Fe and Cu contents in shoots (leaves plus crowns). The DNA obtained from amplification of the FC-R mRNA was cloned and several of the inserts analysed by single strand confirmation polymorphism (SSCP). Although there were different SSCP patterns in the Fe20 treatment, all the inserts that were sequenced were very similar, excluding the hypothesis of more than one FC-R mRNA species being present. The results suggest that Cu as well as Fe is involved in FC-R expression and activity, although the mechanism involved in this regulation is unknown so far. Both small contents of Fe and Cu in plants led to an over-expression of the FC-R gene and enhanced FC-R activity in strawberry roots.
dc.identifier.doihttps://dx.doi.org/10.1080/01904167.2013.816731
dc.identifier.issn0190-4167
dc.identifier.otherAUT: GNO00324; AUT: FPE00387;
dc.identifier.urihttp://hdl.handle.net/10400.1/6225
dc.language.isoeng
dc.peerreviewedyes
dc.publisherTaylor & Francis
dc.relation.isbasedonP-006-FKG
dc.titleRoot ferric chelate reductase is regulated by iron and copper in straberry plants
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage2047
oaire.citation.startPage2035
oaire.citation.titleJournal of Plant Nutrition
oaire.citation.volume36
person.familyNamePestana
person.familyNameCorreia
person.familyNameSaavedra
person.familyNameGama
person.familyNameNolasco
person.givenNameMaribela
person.givenNamePedro José
person.givenNameTeresa
person.givenNameFlorinda
person.givenNameGustavo
person.identifier74793
person.identifier.ciencia-id791E-9EC5-55C9
person.identifier.ciencia-id2E15-3704-0F55
person.identifier.ciencia-id3F1F-53D6-1014
person.identifier.ciencia-idB61D-C9B5-8AE2
person.identifier.orcid0000-0002-4319-1682
person.identifier.orcid0000-0003-4927-1189
person.identifier.orcid0000-0002-2125-6342
person.identifier.orcid0000-0001-6948-0055
person.identifier.orcid0000-0002-7025-6023
person.identifier.ridF-5741-2010
person.identifier.scopus-author-id7005146039
person.identifier.scopus-author-id7006210686
person.identifier.scopus-author-id36467141800
person.identifier.scopus-author-id35770492700
person.identifier.scopus-author-id6602625528
rcaap.rightsrestrictedAccess
rcaap.typearticle
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relation.isAuthorOfPublication.latestForDiscoveryb61cfa99-52bb-401a-bdbb-084ff6afe23f

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