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Response of key stress-related genes of the seagrass Posidonia oceanica in the vicinity of submarine volcanic vents

2015Journal article Open access
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dc.contributor.authorLauritano, C.
dc.contributor.authorRuocco, M.
dc.contributor.authorDattolo, E.
dc.contributor.authorBuia, M. C.
dc.contributor.authorSilva, João
dc.contributor.authorSantos, Rui
dc.contributor.authorOlivé, Irene
dc.contributor.authorCosta, M. M.
dc.contributor.authorProcaccini, G.
dc.date.accessioned2018-12-07T14:58:26Z
dc.date.available2018-12-07T14:58:26Z
dc.date.issued2015
dc.description.abstractSubmarine volcanic vents are being used as natural laboratories to assess the effects of increased ocean acidity and carbon dioxide (CO2) concentration on marine organisms and communities. However, in the vicinity of volcanic vents other factors in addition to CO2, which is the main gaseous component of the emissions, may directly or indirectly confound the biota responses to high CO2. Here we used for the first time the expression of antioxidant and stress-related genes of the seagrass Posidonia oceanica to assess the stress levels of the species. Our hypothesis is that unknown factors are causing metabolic stress that may confound the putative effects attributed to CO2 enrichment only. We analyzed the expression of 35 antioxidant and stress-related genes of P. oceanica in the vicinity of submerged volcanic vents located in the islands of Ischia and Panarea, Italy, and compared them with those from control sites away from the influence of vents. Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) was used to characterize gene expression patterns. Fifty-one percent of genes analyzed showed significant expression changes. Metal detoxification genes were mostly down-regulated in relation to controls at both Ischia and Panarea, indicating that P. oceanica does not increase the synthesis of heavy metal detoxification proteins in response to the environmental conditions present at the two vents. The up-regulation of genes involved in the free radical detoxification response (e.g., CAPX, SODCP and GR) indicates that, in contrast with Ischia, P. oceanica at the Panarea site faces stressors that result in the production of reactive oxygen species, triggering antioxidant responses. In addition, heat shock proteins were also activated at Panarea and not at Ischia. These proteins are activated to adjust stress-accumulated misfolded proteins and prevent their aggregation as a response to some stressors, not necessarily high temperature. This is the first study analyzing the expression of target genes in marine plants living near natural CO2 vents. Our results call for contention to the general claim of seagrasses as "winners" in a high-CO2 world, based on observations near volcanic vents. Careful consideration of factors that are at play in natural vents sites other than CO2 and acidification is required. This study also constitutes a first step for using stress-related genes as indicators of environmental pressures in a changing ocean.
dc.description.sponsorshipproject HighGrass "High-CO2 effects on seagrass photosynthetic ecophysiology" [PTDC/MAREST/3687/2012]; MIUR Italian flagship project RITMARE; ESF COST Action "Seagrass Productivity: from genes to ecosystem management"
dc.identifier.doi10.5194/bg-12-4185-2015
dc.identifier.issn1726-4170
dc.identifier.issn1726-4189
dc.identifier.urihttp://hdl.handle.net/10400.1/12021
dc.language.isoeng
dc.peerreviewedyes
dc.publisherCopernicus Gesellschaft Mbh
dc.subjectEelgrass zostera-marina
dc.subjectHeat-shock proteins
dc.subjectClimate-change
dc.subjectOxidative stress
dc.subjectCoastal habitats
dc.subjectPeroxiredoxin-Q
dc.subjectSea-uchins
dc.subjectCo2 vents
dc.subjectAcidification
dc.subjectPh
dc.titleResponse of key stress-related genes of the seagrass Posidonia oceanica in the vicinity of submarine volcanic vents
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage4194
oaire.citation.issue13
oaire.citation.startPage4185
oaire.citation.titleBiogeosciences
oaire.citation.volume12
person.familyNameSilva
person.familyNameSantos
person.familyNameOlivé
person.givenNameJoão
person.givenNameRui
person.givenNameIrene
person.identifier82814
person.identifier42560
person.identifier.ciencia-id8216-527E-1BD4
person.identifier.ciencia-id9B18-444D-2244
person.identifier.orcid0000-0002-7211-1661
person.identifier.orcid0000-0002-7861-4366
person.identifier.orcid0000-0002-4865-923X
person.identifier.ridJ-7874-2013
person.identifier.ridB-4168-2008
person.identifier.ridJ-3922-2013
person.identifier.scopus-author-id7403023547
person.identifier.scopus-author-id7201375018
person.identifier.scopus-author-id16242114500
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication61040b53-b17a-40f6-b42e-f5476a7a6ddb
relation.isAuthorOfPublication84487ca8-404a-4584-81f0-3662c340d712
relation.isAuthorOfPublicationf4552640-d88e-4323-b28a-822c85f533ff
relation.isAuthorOfPublication.latestForDiscovery84487ca8-404a-4584-81f0-3662c340d712

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