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Microsatellite markers developed through pyrosequencing allow clonal discrimination in the invasive alga Caulerpa taxifolia

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dc.contributor.authorARNAUD-HAOND, Sophie
dc.contributor.authorCandeias, Rui
dc.contributor.authorSerrão, Ester
dc.contributor.authorTeixeira, Sara
dc.date.accessioned2014-05-23T12:31:32Z
dc.date.available2014-05-23T12:31:32Z
dc.date.issued2013
dc.date.updated2014-05-21T11:24:28Z
dc.description.abstractPolymorphic microsatellites were developed for the invasive green algae Caulerpa taxifolia using next generation DNA sequencing. Results showed a limited rate of microsatellites for the amount of sequences, possibly explaining failure of previous attempts for microsatellite development through classical methods. Eight polymorphic loci were selected that exhibited polymorphism and a null or negligible rate of amplification failure. The number of alleles per locus ranged from two to seven. The reconstruction of Multi Locus Genotypes and the heterozygosity and departure from Hardy–Weinberg equilibrium confirmed the influence of clonal reproduction and showed the usefulness of this set of markers to successfully discriminate clonal lineages and analyze the clonal and genetic composition of algal beds. These markers will be used to further investigate the clonal composition and genetic structure in populations of C. taxifolia and to attempt retracing the origin of and pathways followed by invasive clonal lineages.por
dc.identifier.citationArnaud-Haond, S.; Candeias, R.; Serrão, E.A.; Teixeira, S.J.L. Microsatellite markers developed through pyrosequencing allow clonal discrimination in the invasive alga Caulerpa taxifolia, Conservation Genetics Resources, 5, 3, 667-669, 2013.por
dc.identifier.doihttp://dx.doi.org/10.1007/s12686-013-9878-8
dc.identifier.issn1877-7252
dc.identifier.otherAUT: ESE00527;
dc.identifier.urihttp://hdl.handle.net/10400.1/4047
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherSpringerpor
dc.subjectCaulerpa taxifoliapor
dc.subjectAlgaepor
dc.subjectInvasionpor
dc.subjectMediterraneanpor
dc.subjectQueenslandpor
dc.subjectNew South Walespor
dc.subjectSeagrasspor
dc.titleMicrosatellite markers developed through pyrosequencing allow clonal discrimination in the invasive alga Caulerpa taxifoliapor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage669por
oaire.citation.issue3por
oaire.citation.startPage667por
oaire.citation.titleConservation Genetics Resourcespor
oaire.citation.volume5por
person.familyNameARNAUD-HAOND
person.familyNameSerrao
person.familyNameTeixeira
person.givenNameSophie
person.givenNameEster A.
person.givenNameSara
person.identifierC-6686-2012
person.identifier.ciencia-id5A15-FF67-4075
person.identifier.ciencia-id5B13-B26E-B1EC
person.identifier.orcid0000-0001-5814-8452
person.identifier.orcid0000-0003-1316-658X
person.identifier.orcid0000-0003-0419-2348
person.identifier.ridM-3424-2013
person.identifier.scopus-author-id6602532118
person.identifier.scopus-author-id7004093604
person.identifier.scopus-author-id23986333100
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication2b10c79a-8ca9-4449-ba02-0c240f00d921
relation.isAuthorOfPublication45ccfe90-155c-4d6f-9e86-8f0fd064005f
relation.isAuthorOfPublicationb461f1bb-dfdb-410f-b579-647fa97957ca
relation.isAuthorOfPublication.latestForDiscovery45ccfe90-155c-4d6f-9e86-8f0fd064005f

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