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Strategies to optimize biosensors based on impedance spectroscopy to detect phytic acid using layer-by-layer films

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dc.contributor.authorMoraes, M. L.
dc.contributor.authorMaki, R. M.
dc.contributor.authorPaulovich, F. V.
dc.contributor.authorRodrigues Filho, U. P.
dc.contributor.authorDe Oliveira, M. C. F.
dc.contributor.authorRiul, A.
dc.contributor.authorDe Souza, N. C.
dc.contributor.authorFerreira, M.
dc.contributor.authorGomes, Henrique L.
dc.contributor.authorOliveira, O. N.
dc.date.accessioned2013-12-17T12:00:38Z
dc.date.available2013-12-17T12:00:38Z
dc.date.issued2010
dc.date.updated2013-12-16T22:58:30Z
dc.description.abstractImpedance spectroscopy has been proven a powerful tool for reaching high sensitivity in sensor arrays made with nanostructured films in the so-called electronic tongue systems, whose distinguishing ability may be enhanced with sensing units capable of molecular recognition. In this study we show that for optimized sensors and biosensors the dielectric relaxation processes involved in impedance measurements should also be considered, in addition to an adequate choice of sensing materials. We used sensing units made from layer-by-layer (LbL) films with alternating layers of the polyeletrolytes, poly(allylamine) hydrochloride (PAH) and poly(vinyl sulfonate) (PVS), or LbL films of PAH alternated with layers of the enzyme phytase, all adsorbed on gold interdigitate electrodes. Surprisingly, the detection of phytic acid was as effective in the PVS/PAH sensing system as with the PAH/phytase system, in spite of the specific interactions of the latter. This was attributed to the dependence of the relaxation processes on nonspecific interactions such as electrostatic cross-linking and possibly on the distinct film architecture as the phytase layers were found to grow as columns on the LbL film, in contrast to the molecularly thin PAH/PVS films. Using projection techniques, we were able to detect phytic acid at the micromolar level with either of the sensing units in a data analysis procedure that allows for further optimization.por
dc.identifier.citationMoraes, M. L.; Maki, R. M.; Paulovich, F. V.; Rodrigues Filho, U. P.; De Oliveira, M. C. F.; Riul, A.; De Souza, N. C.; Ferreira, M.; Gomes, H. L.; Oliveira, O. N. Strategies to optimize biosensors based on impedance spectroscopy to detect phytic acid using layer-by-layer films, Analytical Chemistry, 82, 8, 3239-32, 2010.por
dc.identifier.doihttp://dx.doi.org/10.1021/ac902949h
dc.identifier.issn0003-2700
dc.identifier.otherAUT: HGO00803;
dc.identifier.urihttp://hdl.handle.net/10400.1/3257
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherAmerican Chemical Societypor
dc.titleStrategies to optimize biosensors based on impedance spectroscopy to detect phytic acid using layer-by-layer filmspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage3246por
oaire.citation.issue8por
oaire.citation.startPage3239por
oaire.citation.titleAnalytical Chemistrypor
oaire.citation.volume82por
person.familyNameGomes
person.givenNameHenrique Leonel
person.identifier.orcid0000-0003-3664-4740
person.identifier.scopus-author-id7005305880
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication6da677b9-927f-423d-8657-448a0dccb67c
relation.isAuthorOfPublication.latestForDiscovery6da677b9-927f-423d-8657-448a0dccb67c

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