The use of enhanced green fluorescent protein to track matrix Gla protein expression during zebrafish (Danio rerio) development

Cardeira Da Silva, João; Gavaia, Paulo J.; Brito, A. B.; Cancela, Leonor

http://hdl.handle.net/10400.1/4353

Use this identifier to reference this record.

Name:	Description:	Size:	Format:
The use of enhance green fluorescent protein.pdf		1.97 MB	Adobe PDF	Download

Send Feedback

Authors

Cardeira Da Silva, João

Gavaia, Paulo J.

Brito, A. B.

Cancela, Leonor

Abstract(s)

Matrix Gla protein (mgp), a vitamin K-dependent protein, is an important element of skeleton development as it regulates mineralization of both cartilaginous and bony structures, among others. Although mgp is found in a wide range of tissues, it is known to be expressed mostly by chondrocytes and vascular smooth muscle cells. Accordingly, inhibition of Mgp function results in over-mineralization of cartilages and growth plates and in ectopic mineralizations, particularly in the vascular system. Besides being a well characterized protein and the existence of developmental studies in zebrafish, there is still no description of monitoring mgp expression in live specimens.

URI

http://hdl.handle.net/10400.1/4353

Citation

Cardeira da Silva J., Gavaia P.J., Brito A.B., Cancela M.L. 2008. The use of enhanced green fluorescent protein to track matrix Gla protein expression during zebrafish (Danio rerio) development. Marine Genomics Europe final meeting. CCMAR-University of Algarve May 13-16, Faro, Portugal.