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Impact of temperature on Phenolic and Osmolyte contents in In Vitro cultures and micropropagated plants of two mediterranean plant species, Lavandula viridis and Thymus lotocephalus

2022-12-14Journal article Open Access
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dc.contributor.authorMansinhos, Inês
dc.contributor.authorGonçalves, Sandra
dc.contributor.authorRodríguez Solana, Raquel
dc.contributor.authorOrdóñez-Díaz, José Luis
dc.contributor.authorMoreno-Rojas, José Manuel
dc.contributor.authorRomano, Anabela
dc.date.accessioned2023-01-06T11:35:42Z
dc.date.available2023-01-06T11:35:42Z
dc.date.issued2022-12-14
dc.date.updated2022-12-22T14:36:19Z
dc.description.abstractIn this study, in vitro cultures and micropropagated plants of two Mediterranean aromatic plants, Lavandula viridis L’Hér and Thymus lotocephalus López and Morales, were exposed to different temperatures (15, 20, 25, and 30 ◦C). The effect of temperature on the levels of hydrogen peroxide (H2O2 ), lipid peroxidation, and osmoprotectants (proline, soluble sugars, and soluble proteins), as well as on the phenolic profile by HPLC-HRMS and intermediates of the secondary metabolism (phenylalanine ammonia lyase (PAL) activity and shikimic acid content), was investigated. Moreover, the antioxidant activity of the plant extracts was also analyzed. Overall, considering the lipid peroxidation and H2O2 content, the extreme temperatures (15 and 30 ◦C) caused the greatest damage to both species, but the osmoprotectant response was different depending on the species and plant material. In both species, phenolic compounds and related antioxidant activity increased with the rise in temperature in the micropropagated plants, while the opposite occurred in in vitro cultures. L. viridis cultures showed the highest biosynthesis of rosmarinic acid (92.6 g/kgDW) at 15 ◦C and seem to be a good alternative to produce this valuable compound. We conclude that contrasting temperatures greatly influence both species’ primary and secondary metabolism, but the response is different depending on the plant micropropagation stage.pt_PT
dc.description.sponsorshipPOSTDOC_21_00914
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPlants 11 (24): 3516 (2022)pt_PT
dc.identifier.doi10.3390/plants11243516pt_PT
dc.identifier.eissn2223-7747
dc.identifier.urihttp://hdl.handle.net/10400.1/18742
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationMediterranean Institute for Agriculture, Environment and Development
dc.relationImpact of challenging environmental conditions on plant secondary metabolism
dc.relationCHANGE - Global Change and Sustainability Institute
dc.relationNot Available
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAbiotic stresspt_PT
dc.subjectOsmolytespt_PT
dc.subjectShikimate/phenylpropanoid pathwaypt_PT
dc.subjectGreen extractpt_PT
dc.subjectNADESpt_PT
dc.subjectAntioxidant activitypt_PT
dc.subjectPhenolic acidspt_PT
dc.titleImpact of temperature on Phenolic and Osmolyte contents in In Vitro cultures and micropropagated plants of two mediterranean plant species, Lavandula viridis and Thymus lotocephaluspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMediterranean Institute for Agriculture, Environment and Development
oaire.awardTitleImpact of challenging environmental conditions on plant secondary metabolism
oaire.awardTitleCHANGE - Global Change and Sustainability Institute
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F05183%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F145243%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0121%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC INST 2ed/CEECINST%2F00052%2F2021%2FCP2792%2FCT0001/PT
oaire.citation.issue24pt_PT
oaire.citation.startPage3516pt_PT
oaire.citation.titlePlantspt_PT
oaire.citation.volume11pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamCEEC INST 2ed
person.familyNamePaixão Mansinhos
person.familyNameGonçalves
person.familyNameRodríguez Solana
person.familyNameRomano
person.givenNameInês Filipa
person.givenNameSandra
person.givenNameRaquel
person.givenNameAnabela
person.identifier624004
person.identifier.ciencia-id4114-8891-54E1
person.identifier.ciencia-id9F19-E3AE-2C59
person.identifier.ciencia-idFE10-7889-0562
person.identifier.ciencia-id8A18-7E9A-3B0E
person.identifier.orcid0000-0001-9632-8876
person.identifier.orcid0000-0002-3038-4434
person.identifier.orcid0000-0003-4204-7387
person.identifier.orcid0000-0002-7204-7428
person.identifier.ridM-6988-2017
person.identifier.ridI-6317-2015
person.identifier.scopus-author-id8583003200
person.identifier.scopus-author-id55081917100
person.identifier.scopus-author-id56249997600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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