Loading...
Publication
Impact of temperature on Phenolic and Osmolyte contents in In Vitro cultures and micropropagated plants of two mediterranean plant species, Lavandula viridis and Thymus lotocephalus
| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 4.85 MB | Adobe PDF |
Advisor(s)
Abstract(s)
In this study, in vitro cultures and micropropagated plants of two Mediterranean aromatic
plants, Lavandula viridis L’Hér and Thymus lotocephalus López and Morales, were exposed to different
temperatures (15, 20, 25, and 30 ◦C). The effect of temperature on the levels of hydrogen peroxide
(H2O2
), lipid peroxidation, and osmoprotectants (proline, soluble sugars, and soluble proteins), as
well as on the phenolic profile by HPLC-HRMS and intermediates of the secondary metabolism
(phenylalanine ammonia lyase (PAL) activity and shikimic acid content), was investigated. Moreover,
the antioxidant activity of the plant extracts was also analyzed. Overall, considering the lipid
peroxidation and H2O2 content, the extreme temperatures (15 and 30 ◦C) caused the greatest damage
to both species, but the osmoprotectant response was different depending on the species and plant
material. In both species, phenolic compounds and related antioxidant activity increased with the
rise in temperature in the micropropagated plants, while the opposite occurred in in vitro cultures.
L. viridis cultures showed the highest biosynthesis of rosmarinic acid (92.6 g/kgDW) at 15 ◦C and
seem to be a good alternative to produce this valuable compound. We conclude that contrasting
temperatures greatly influence both species’ primary and secondary metabolism, but the response is
different depending on the plant micropropagation stage.
Description
Keywords
Abiotic stress Osmolytes Shikimate/phenylpropanoid pathway Green extract NADES Antioxidant activity Phenolic acids
Citation
Plants 11 (24): 3516 (2022)
Publisher
MDPI