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Regulation of transthyretin by thyroid hormones in Wsh

2007Journal article Restricted access
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dc.contributor.authorMorgado, Isabel
dc.contributor.authorSantos, C. R. A.
dc.contributor.authorJacinto, R.
dc.contributor.authorPower, Deborah
dc.date.accessioned2014-10-23T10:33:53Z
dc.date.available2014-10-23T10:33:53Z
dc.date.issued2007
dc.description.abstractTransthyretin (TTR) is a thyroid hormone-binding protein (THBP) which in its tetrameric form transports thyroid hormones (THs), thyroxine (T4) and triiodothyronine (T3) in the blood of vertebrates. The principal site of production of TTR is the liver but in the sea bream TTR mRNA is also present in the heart, intestine and brain. The regulation of TTR is unstudied in Wsh and the normal circulating level of this THBP is unknown. The aim of the present study was to establish factors which regulate TTR production in Wsh. As a Wrst step a number of tools were generated; sea bream recombinant TTR (sbrTTR) and speciWc sbrTTR antisera which were used to establish an ELISA (enzyme-linked immunosorbent assay) for measuring TTR plasma levels. Subsequently, an experiment was conducted to determine the inXuence of THs on TTR production. Circulating physiological levels of TTR in sea bream determined by ELISA are approximately 3.8 gml¡1. Administration of T3 and T4 to sea bream signiWcantly increased (p< 0.001 and p<0.005, respectively) the concentration of circulating TTR (V11.5 gml¡1) in relation to control Wsh, but did not change gene transcription in the liver. Methimazol (MMI) an antithyroid agent, failed to signiWcantly reduce circulating THs below control levels but signiWcantly increased (p < 0.005) plasma TTR levels (approximately 10.8 gml¡1) and decreased (p< 0.05) transcription in the liver. Future studies will aim to elucidate in more detail these regulatory pathways.por
dc.description.sponsorshipWe thank Dr. Pep Rotllant for assistance in establishing the ELISA. Work co-Wnanced by POCI 2010 and the European social funds attributed by the Portuguese National Science Foundation (FCT) to project POCTI/CVT/38703/ 2001, Pluriannual project to CCMAR and a PhD fellowship to I.M. (SFRH/BD/6091/2001).por
dc.identifier.citationI. Morgado, C.R.A. Santos, R. Jacinto, D.M. Power, "Regulation of transthyretin by thyroid hormones in Wsh" in General and Comparative Endocrinology 152 (2007) 189–197.por
dc.identifier.doihttp://dx.doi.org/10.1016/j.ygcen.2006.12.017
dc.identifier.issn0016-6480
dc.identifier.otherAUT: DPO00386;
dc.identifier.urihttp://hdl.handle.net/10400.1/5425
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectPlasma transthyretinpor
dc.subjectSea breampor
dc.subjectThyroid hormonespor
dc.subjectTransthyretin regulationpor
dc.subjectTTR Elisapor
dc.titleRegulation of transthyretin by thyroid hormones in Wshpor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage197por
oaire.citation.issue2-3por
oaire.citation.startPage189por
oaire.citation.titleGeneral and Comparative Endocrinologypor
oaire.citation.volume152por
person.familyNameMorgado
person.familyNamePower
person.givenNameIsabel
person.givenNameDeborah Mary
person.identifier.ciencia-id3315-7715-C3B4
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0002-7826-997X
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridJ-4798-2013
person.identifier.scopus-author-id18134268300
person.identifier.scopus-author-id7101806760
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication67026aa2-ccdb-4b68-9938-b09e0fd6aca0
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscovery67026aa2-ccdb-4b68-9938-b09e0fd6aca0

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