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Incubation temperature induces changes in muscle cellularity and gene expression in Senegalese sole (Solea senegalensis)

2013Journal article Restricted access
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dc.contributor.authorCampos, C.
dc.contributor.authorValente, L. M. P.
dc.contributor.authorConceição, L. E. C.
dc.contributor.authorEngrola, S.
dc.contributor.authorSousa, V.
dc.contributor.authorRocha, E.
dc.contributor.authorFernandes, J. M. O.
dc.date.accessioned2014-07-03T09:10:41Z
dc.date.available2014-07-03T09:10:41Z
dc.date.issued2013
dc.date.updated2014-06-27T13:52:56Z
dc.description.abstractFertilised eggs of Senegalese sole were incubated at 15, 18 or 21 °C, and after hatching all larvae were reared at 21 °C until 30 days post-hatch. By this point larvae from the 18 or 21 °C temperature groups had 11 and 9% more muscle fibres than those from 15 °C, respectively. Hyperplastic growth during metamorphosis was higher in larvae from 18 °C. Embryonic temperature induced gene expression changes, albeit with a variable pattern throughout development. Myf5, myod2, myHC and fst mRNA levels were significantly higher at several stages prior to hatching in embryos incubated at 21 °C, whereas hsp90AB and hsp70 transcripts were present at higher levels in the 15 °C group. Myf5, myod1, myod2, pax7, myog, fst, igf-II, igf1r, hsp90AA and hsp90AB were expressed at higher levels during early development, particularly during somitogenesis. In contrast, mrf4, myHC, mylc2, igf-I, mstn1 and hsp70 were up-regulated at later stages of larval development, namely during and after metamorphosis. This study is the first example of thermal plasticity of myogenesis with prolonged effect in a flatfish.por
dc.identifier.citationCampos, C.; Valente, L.M.P.; Conceição, L.E.C.; Engrola, S.; Sousa, V.; Rocha, E.; Fernandes, J.M.O.Incubation temperature induces changes in muscle cellularity and gene expression in Senegalese sole (Solea senegalensis), Gene, 516, 2, 209-217, 2013.por
dc.identifier.doihttp://dx.doi.org/ 10.1016/j.gene.2012.12.074
dc.identifier.issn1879-0038
dc.identifier.urihttp://hdl.handle.net/10400.1/4628
dc.language.isoengpor
dc.peerreviewedyespor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0378111912016332por
dc.subjectSolea senegalensispor
dc.subjectEpigeneticspor
dc.subjectDevelopmentpor
dc.subjectMuscle cellularitypor
dc.subjectThermal plasticitypor
dc.subjectMyogenesispor
dc.titleIncubation temperature induces changes in muscle cellularity and gene expression in Senegalese sole (Solea senegalensis)por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage217por
oaire.citation.issue2por
oaire.citation.startPage209por
oaire.citation.titleGenepor
oaire.citation.volume516por
person.familyNameEngrola
person.givenNameSofia
person.identifier237846
person.identifier.ciencia-idA610-29E7-9B83
person.identifier.orcid0000-0002-5244-5541
person.identifier.ridA-2485-2012
person.identifier.scopus-author-id8669620600
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication51be5a20-df9c-4a90-85eb-51d779642caa
relation.isAuthorOfPublication.latestForDiscovery51be5a20-df9c-4a90-85eb-51d779642caa

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