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Selective elimination of chloroplastidial dna for metagenomics of bacteria associated with the green alga caulerpa taxifolia (bryopsidophyceae)

2012Journal article Restricted access
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dc.contributor.authorAires, Tânia
dc.contributor.authorMarbà, N.
dc.contributor.authorSerrão, Ester
dc.contributor.authorDuarte, C. M.
dc.contributor.authorARNAUD-HAOND, Sophie
dc.date.accessioned2014-05-28T13:38:34Z
dc.date.available2014-05-28T13:38:34Z
dc.date.issued2012
dc.date.updated2014-05-21T11:37:53Z
dc.description.abstractMolecular analyses of bacteria associated with photosynthetic organisms are often confounded by coamplification of the chloroplastidial 16S rDNA with the targeted bacterial 16S rDNA. This major problem has hampered progress in the characterization of bacterial communities associated to photo-synthetic organisms and has limited the full realization of the potential offered by the last generation of metagenomics approaches. A simple and inexpensive method is presented, based on ethanol and bleach treatments prior to extraction, to efficiently discard a great part of chloroplastidial DNA without affecting the characterization of bacterial communities through pyrosequencing. Its effectiveness for the description of bacterial lineages associated to the green alga Caulerpa taxifolia (M. Vahl) C. Agardh was much higher than that of the preexisting enrichment protocols proposed for plants. Furthermore, this new technique requires a very small amount of biological material compared to the other current protocols, making it more realistic for systematic use in ecological and phylogenetic studies and opening promising prospects for metagenomics of green algae, as shown by our data.por
dc.identifier.citationAires, T.; Marbà, N.; Serrao, E.A.; Duarte, C.M.; Arnaud-Haond, S.Selective elimination of chloroplastidial dna for metagenomics of bacteria associated with the green alga caulerpa taxifolia (bryopsidophyceae), Journal of Phycology, 48, 2, 483-490, 2012por
dc.identifier.doihttp://dx.doi.org/10.1111/j.1529-8817.2012.01124.x
dc.identifier.issn0022-3646
dc.identifier.otherAUT: ESE00527;
dc.identifier.urihttp://hdl.handle.net/10400.1/4107
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherPhycological Society of Americapor
dc.subject16Spor
dc.subjectEliminationpor
dc.subjectChloroplastspor
dc.subjectMetagenomicspor
dc.subjectGreen algaepor
dc.titleSelective elimination of chloroplastidial dna for metagenomics of bacteria associated with the green alga caulerpa taxifolia (bryopsidophyceae)por
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage490por
oaire.citation.startPage483por
oaire.citation.titleJournal of Phycologypor
oaire.citation.volume48por
person.familyNameAires
person.familyNameSerrao
person.familyNameARNAUD-HAOND
person.givenNameTania
person.givenNameEster A.
person.givenNameSophie
person.identifier282868
person.identifierC-6686-2012
person.identifier.ciencia-id4318-9E2C-32B9
person.identifier.ciencia-id5B13-B26E-B1EC
person.identifier.ciencia-id5A15-FF67-4075
person.identifier.orcid0000-0002-1964-6819
person.identifier.orcid0000-0003-1316-658X
person.identifier.orcid0000-0001-5814-8452
person.identifier.ridM-8306-2013
person.identifier.scopus-author-id7004093604
person.identifier.scopus-author-id6602532118
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationbb3e6904-8ea3-43e6-8c43-4979daafec9b
relation.isAuthorOfPublication45ccfe90-155c-4d6f-9e86-8f0fd064005f
relation.isAuthorOfPublication2b10c79a-8ca9-4449-ba02-0c240f00d921
relation.isAuthorOfPublication.latestForDiscovery2b10c79a-8ca9-4449-ba02-0c240f00d921

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