Repository logo
 
Publication

Interactions of vanadium(V)–citrate complexes with the sarcoplasmic reticulum calcium pump

2005Journal article Open access
Simple item page
dc.contributor.authorAureliano, M.
dc.contributor.authorTiago, Teresa
dc.contributor.authorGândara, Ricardo M. C.
dc.contributor.authorSousa, Andrea
dc.contributor.authorModerno, A.
dc.contributor.authorKaliva, M.
dc.contributor.authorSalifoglou, A.
dc.contributor.authorDuarte, Rui O.
dc.contributor.authorMoura, José J. G.
dc.date.accessioned2012-06-26T10:16:13Z
dc.date.available2012-06-26T10:16:13Z
dc.date.issued2005
dc.description.abstractAmong the biotargets interacting with vanadium is the calcium pump from the sarcoplasmic reticulum (SR). To this end, initial research efforts were launched with two vanadium(V)–citrate complexes, namely (NH4)6[V2O4(C6H4O7)2] Æ 6H2O and (NH4)6[V2O2(O2)2(C6H4O7)2] Æ 4H2O, potentially capable of interacting with the SR calcium pump by combining kinetic studies with 51V NMR spectroscopy. Upon dissolution in the reaction medium (concentration range: 4–0.5 mM), both vanadium(V):citrate (VC) and peroxovanadium(V):citrate (PVC) complexes are partially converted into vanadate oligomers. A 1 mM solution of the PVC complex, containing 184 lM of the PVC complex, 94 lM oxoperoxovanadium(V) (PV) species, 222 lM monomeric (V1), 43 lM dimeric (V2) and 53 lM tetrameric (V4) species, inhibits Ca2+ accumulation by 75 %, whereas a solution of the VC complex of the same vanadium concentration, containing 98 lM of the VC complex, 263 lM monomeric (V1), 64 lM dimeric (V2) and 92 lM tetrameric (V4) species inhibits the calcium pump activity by 33 %. In contrast, a 1 mM metavanadate solution, containing 460 lM monomeric (V1), 90.2 lM dimeric (V2) and 80 lM tetrameric (V4) species, has no effect on Ca2+ accumulation. The NMR signals from the VC complex ( 548.0 ppm), PVC complex ( 551.5 ppm) and PV ( 611.1 ppm) are broadened upon SR vesicle addition (2.5 mg/ml total protein). The relative order for the half width line broadening of the NMR signals, which reflect the interaction with the protein, was found to be V4 > PVC > VC > PV > V2 = V1 = 1, with no effect observed for the V1 and V2 signals. Putting it all together the effects of two vanadium( V)–citrate complexes on the modulation of calcium accumulation and ATP hydrolysis by the SR calcium pump reflected the observed variable reactivity into the nature of key species forming upon dissolution of the title complexes in the reaction media.por
dc.identifier.issn0162-0134
dc.identifier.urihttp://hdl.handle.net/10400.1/1291
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectCanadium complexespor
dc.subjectCalcium pumppor
dc.titleInteractions of vanadium(V)–citrate complexes with the sarcoplasmic reticulum calcium pumppor
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F2924%2F2000/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F8912%2F2002/PT
oaire.citation.endPage2361por
oaire.citation.issue99por
oaire.citation.startPage2355por
oaire.citation.titleJournal of Inorganic Biochemistrypor
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
person.familyNameAureliano
person.givenNameManuel
person.identifier584146
person.identifier.ciencia-idAA14-3490-DC5E
person.identifier.orcid0000-0003-4858-3201
person.identifier.ridI-3283-2012
person.identifier.scopus-author-id6603412860
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationbb413661-7edd-4b57-8338-33889cfd05db
relation.isAuthorOfPublication.latestForDiscoverybb413661-7edd-4b57-8338-33889cfd05db
relation.isProjectOfPublicationba989a5c-aa42-43bc-94cd-956398eda40b
relation.isProjectOfPublicationcae2b781-f8d0-4352-8a55-50bc022fae57
relation.isProjectOfPublication.latestForDiscoveryba989a5c-aa42-43bc-94cd-956398eda40b

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
AurelianoArt.JIB2005c.pdf
Size:
194.28 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

FCT2-Artigos (em revistas ou actas indexadas)