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Screening of mineralogenic and osteogenic compounds in zebrafish—tools to improve assay throughput and data accuracy

2022-08-10Journal article Open access
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dc.contributor.authorRosa, Joana
dc.contributor.authorTarasco, Marco
dc.contributor.authorJ. Gavaia, Paulo
dc.contributor.authorLeonor Cancela, M.
dc.contributor.authorLaizé, Vincent
dc.date.accessioned2022-08-31T12:54:50Z
dc.date.available2022-08-31T12:54:50Z
dc.date.issued2022-08-10
dc.date.updated2022-08-25T11:17:59Z
dc.description.abstractBone disorders affect millions of people worldwide and treatments currently available often produce undesirable secondary effects or have limited efficacy. It is therefore of the utmost interest for patients to develop more efficient drugs with reduced off-target activities. In the long process of drug development, screening and preclinical validation have recently gained momentum with the increased use of zebrafish as a model organism to study pathological processes related to human bone disorders, and the development of zebrafish high-throughput screening assays to identify bone anabolic compounds. In this review, we provided a comprehensive overview of the literature on zebrafish bone-related assays and evaluated their performance towards an integration into screening pipelines for the discovery of mineralogenic/osteogenic compounds. Tools available to standardize fish housing and feeding procedures, synchronize embryo production, and automatize specimen sorting and image acquisition/analysis toward faster and more accurate screening outputs were also presented.pt_PT
dc.description.sponsorshipPT ALG-01-0145-FEDER-022121; POCI-01-0247-FEDER-047200; PERFORMALGAE ALG-01-0247-FEDER-06996; OSTEOMAR 16.02.01-FMP-0057; BLUEHUMAN EAPA_151/2016; PT ALG-01-0145-FEDER-022121; POCI-01-0247-FEDER-047200; PERFORMALGAE ALG-01-0247-FEDER-06996
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPharmaceuticals 15 (8): 983 (2022)pt_PT
dc.identifier.doi10.3390/ph15080983pt_PT
dc.identifier.eissn1424-8247
dc.identifier.urihttp://hdl.handle.net/10400.1/18198
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relationHow microplastics and adsorbed pollutants affect zebrafish development
dc.relationHow microplastics and adsorbed pollutants affect zebrafish development
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectDrug discoverypt_PT
dc.subjectScreening pipelinept_PT
dc.subjectBone anabolic compoundspt_PT
dc.subjectZebrafish Danio reriopt_PT
dc.subjectTechnological innovationpt_PT
dc.subjectHigh throughputpt_PT
dc.titleScreening of mineralogenic and osteogenic compounds in zebrafish—tools to improve assay throughput and data accuracypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardTitleHow microplastics and adsorbed pollutants affect zebrafish development
oaire.awardTitleHow microplastics and adsorbed pollutants affect zebrafish development
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F128634%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/COVID%2FBD%2F151848%2F2021/PT
oaire.citation.issue15pt_PT
oaire.citation.startPage983pt_PT
oaire.citation.titlePharmaceuticalspt_PT
oaire.citation.volume8pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
oaire.fundingStreamOE
person.familyNameRosa
person.familyNameTarasco
person.familyNameGavaia
person.familyNameCancela
person.familyNameLaizé
person.givenNameJoana
person.givenNameMarco
person.givenNamePaulo
person.givenNameM. Leonor
person.givenNameVincent
person.identifier.ciencia-id0B18-8AEC-875C
person.identifier.ciencia-id2B1F-0392-9ABE
person.identifier.ciencia-idB619-FC16-D007
person.identifier.orcid0000-0001-7947-2681
person.identifier.orcid0000-0001-5894-6526
person.identifier.orcid0000-0002-9582-1957
person.identifier.orcid0000-0003-3114-6662
person.identifier.orcid0000-0001-9565-9198
person.identifier.ridG-9575-2017
person.identifier.ridA-6470-2011
person.identifier.ridB-4463-2008
person.identifier.scopus-author-id36505067900
person.identifier.scopus-author-id57194105105
person.identifier.scopus-author-id6507104377
person.identifier.scopus-author-id6602982778
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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