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Comparison of RNA/DNA ratios obtained with two methods for nucleic acid quantification in gobiid larvae

2000Journal article Restricted access
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dc.contributor.authorEsteves, E.
dc.contributor.authorChicharo, Alexandra
dc.contributor.authorPina, T.
dc.contributor.authorCoelho, M. L.
dc.contributor.authorAndrade, José Pedro
dc.date.accessioned2013-02-21T15:29:20Z
dc.date.available2013-02-21T15:29:20Z
dc.date.issued2000
dc.date.updated2013-02-19T16:28:53Z
dc.description.abstractIn this paper, RNA and DNA contents and RNA/DNA ratios obtained with two fluorometric methods for the extraction and quantification of nucleic acids in individual estuarine gobiid larvae are compared. A total of 141 gobiid larvae, collected in the Mira and Guadiana estuaries (Portugal), were analysed. This study indicated that the nucleic acids content and RNA/DNA ratios of individual larvae varied with the procedure used. The two methods yielded similar log DNA values but different log RNA and log (RNA/DNA). The differences could be due to a number of factors related to specific steps of tested protocols. Comparison of nucleic acids contents and calibration of the two methods by regression analysis provided a reasonable basis for comparison although differences between estimates were evident.por
dc.identifier.citationEsteves, E.; Chicharo, M. A.; Pina, T.; Coelho, M. L.; Andrade, J. P. Comparison of RNA/DNA ratios obtained with two methods for nucleic acid quantification in gobiid larvae, Journal of Experimental Marine Biology and Ecology, 245, 1, 43-55, 2000.por
dc.identifier.issn0022-0981
dc.identifier.otherAUT: MCH00377;JAN00399;EES01759;
dc.identifier.urihttp://hdl.handle.net/10400.1/2393
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.subjectFluorimetric methodspor
dc.subjectGobiid larvaepor
dc.subjectRNApor
dc.subjectDNA ratiospor
dc.subjectMarine fish larvaepor
dc.subjectRNA-DNA ratiospor
dc.titleComparison of RNA/DNA ratios obtained with two methods for nucleic acid quantification in gobiid larvaepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage55por
oaire.citation.issue1por
oaire.citation.startPage43por
oaire.citation.titleJournal of Experimental Marine Biology and Ecologypor
oaire.citation.volume245por
person.familyNameEsteves
person.familyNameTeodosio
person.familyNameAndrade
person.givenNameEduardo
person.givenNameMaria
person.givenNameJose
person.identifier.ciencia-idC81D-666A-FF24
person.identifier.ciencia-idAF10-647B-65FB
person.identifier.ciencia-idD514-1A21-F020
person.identifier.orcid0000-0002-1872-8185
person.identifier.orcid0000-0002-0939-9885
person.identifier.orcid0000-0002-5482-9776
person.identifier.ridE-9582-2012
person.identifier.ridB-5077-2013
person.identifier.scopus-author-id7003885183
person.identifier.scopus-author-id56196396700
person.identifier.scopus-author-id7201722097
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationaa7fd030-1bcb-4f16-83ad-508346a35f97
relation.isAuthorOfPublicationb39b2d6d-d6f5-4130-a9b9-4e7624d44676
relation.isAuthorOfPublication8f1ffbdc-85aa-4342-8bd8-61eb6d6be74e
relation.isAuthorOfPublication.latestForDiscovery8f1ffbdc-85aa-4342-8bd8-61eb6d6be74e

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