Repository logo
 
Publication

Can macroalgae provide promising anti-tumoral compounds? A closer look at Cystoseira tamariscifolia as a source for antioxidant and anti-hepatocarcinoma compounds

2016-02Journal article Open Access
Simple item page
dc.contributor.authorVizetto-Duarte, C
dc.contributor.authorCustódio, Luísa
dc.contributor.authorAcosta, Gerardo
dc.contributor.authorLago, João H. G.
dc.contributor.authorMorais, Thiago R.
dc.contributor.authorSousa, Carolina Bruno de
dc.contributor.authorGangadhar, Katkam N.
dc.contributor.authorRodrigues, Maria Joao
dc.contributor.authorPereira, Hugo
dc.contributor.authorLima, Raquel T.
dc.contributor.authorVasconcelos, M. Helena
dc.contributor.authorBarreira, Luísa
dc.contributor.authorRauter, Amélia P.
dc.contributor.authorAlbericioi, Fernando
dc.contributor.authorVarela, J.
dc.date.accessioned2017-04-07T15:57:08Z
dc.date.available2017-04-07T15:57:08Z
dc.date.issued2016-02
dc.description.abstractMarine organisms are a prolific source of drug leads in a variety of therapeutic areas. In the last few years, biomedical, pharmaceutical and nutraceutical industries have shown growing interest in novel compounds from marine organisms, including macroalgae. Cystoseira is a genus of Phaeophyceae (Fucales) macroalgae known to contain bioactive compounds. Organic extracts (hexane, diethyl ether, ethyl acetate and methanol extracts) from three Cystoseira species (C. humilis, C. tamariscifolia and C. usneoides) were evaluated for their total phenolic content, radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'- azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radicals, and antiproliferative activity against a human hepatocarcinoma cell line (HepG2 cells). C. tamariscifolia had the highest TPC and RSA. The hexane extract of C. tamariscifolia (CTH) had the highest cytotoxic activity (IC50 = 2.31 mu g/mL), and was further tested in four human tumor (cervical adenocarcinoma HeLa; gastric adenocarcinoma AGS; colorectal adenocarcinoma HCT-15; neuroblastoma SH-SY5Y), and two non-tumor (murine bone marrow stroma S17 and human umbilical vein endothelial HUVEC) cell lines in order to determine its selectivity. CTH strongly reduced viability of all tumor cell lines, especially of HepG2 cells. Cytotoxicity was particularly selective for the latter cells with a selectivity index = 12.6 as compared to non-tumor cells. Incubation with CTH led to a 2-fold decrease of HepG2 cell proliferation as shown by the bromodeoxyuridine (BrdU) incorporation assay. CTH-treated HepG2 cells presented also pro-apoptotic features, such as increased Annexin Wpropidium iodide (PI) binding and dose-dependent morphological alterations in DAPI-stained cells. Moreover, it had a noticeable disaggregating effect on 3D multicellular tumor spheroids. Deme boxy cystoketal chromane, a derivative of the meroditerpenoid cystoketal, was identified as the active compound in CTH and was shown to display selective in vitro cYtotoxicitY towards HepG2 cells.
dc.identifier.doi10.7717/peerj.1704
dc.identifier.issn2167-8359
dc.identifier.otherAUT:JVA01305;
dc.identifier.urihttp://hdl.handle.net/10400.1/9621
dc.language.isoeng
dc.peerreviewedyes
dc.publisherPeerJ
dc.relationMarine photosynthetic organisms of the Algarve coast with biomedical applications
dc.relationHalophytes: a precious resource of nutritional elements and bioactive compounds
dc.relationCentre of Marine Sciences
dc.relationBiomedical properties of Cystoseira species: insights into nutra- and pharmaceutical applications
dc.relationMARINE ALGAE EXTRACTS AS A SOURCE OF NATURAL ANTILEISHMANIAL COMPOUNDS
dc.relationBiotechnological applications of a promising marine chlorophyte Tetraselmis sp. CTP4: a biorefinery approach
dc.relationTECHNOLOGICAL IMPROVEMENTS OF BIODIESEL PRODUCTION USING OILS SYNTHESIZED BY MICROALGAE
dc.relationSMALL MOLECULES AS APOPTOSIS INDUCERS IN CANCER CELLS: INVESTIGATION OF THE MECHANISM OF ACTION
dc.relationBiologically Active Natural Products from Marine Organisms and Salt Marsh Plants (acronym: PharmaBlue)
dc.relation.isbasedonWOS:000370949200004
dc.subjectMarine natural products
dc.subjectBrown algae
dc.subjectAntioxidant
dc.subjectAnti-hepatocarcinoma
dc.subjectCystoseira
dc.titleCan macroalgae provide promising anti-tumoral compounds? A closer look at Cystoseira tamariscifolia as a source for antioxidant and anti-hepatocarcinoma compounds
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMarine photosynthetic organisms of the Algarve coast with biomedical applications
oaire.awardTitleHalophytes: a precious resource of nutritional elements and bioactive compounds
oaire.awardTitleCentre of Marine Sciences
oaire.awardTitleBiomedical properties of Cystoseira species: insights into nutra- and pharmaceutical applications
oaire.awardTitleMARINE ALGAE EXTRACTS AS A SOURCE OF NATURAL ANTILEISHMANIAL COMPOUNDS
oaire.awardTitleBiotechnological applications of a promising marine chlorophyte Tetraselmis sp. CTP4: a biorefinery approach
oaire.awardTitleTECHNOLOGICAL IMPROVEMENTS OF BIODIESEL PRODUCTION USING OILS SYNTHESIZED BY MICROALGAE
oaire.awardTitleSMALL MOLECULES AS APOPTOSIS INDUCERS IN CANCER CELLS: INVESTIGATION OF THE MECHANISM OF ACTION
oaire.awardTitleBiologically Active Natural Products from Marine Organisms and Salt Marsh Plants (acronym: PharmaBlue)
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR%2F103957%2F2008/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FMAR-EST%2F4346%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F04326%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F81425%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F78062%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F105541%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F81882%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F68787%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F00049%2F2012%2FCP0179%2FCT0001/PT
oaire.citation.endPagee1704
oaire.citation.startPagee1704
oaire.citation.titlePeerJ
oaire.citation.volume4
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
oaire.fundingStreamInvestigador FCT
person.familyNameVizetto-Duarte
person.familyNameCustódio
person.familyNameRodrigues
person.familyNamePereira
person.familyNameBarreira
person.familyNameVarela
person.givenNameCatarina
person.givenNameLuísa
person.givenNameMaria João
person.givenNameHugo
person.givenNameLuísa
person.givenNameJoão
person.identifierR-004-VNG
person.identifier282781
person.identifier.ciencia-id791B-C560-AEA2
person.identifier.ciencia-id2514-0E17-1D8D
person.identifier.ciencia-id331E-84FD-2F34
person.identifier.ciencia-id6D1A-17E5-1400
person.identifier.orcid0000-0002-8203-7231
person.identifier.orcid0000-0003-4338-7703
person.identifier.orcid0000-0001-8732-710X
person.identifier.orcid0000-0002-1369-2099
person.identifier.orcid0000-0002-4077-855X
person.identifier.orcid0000-0003-3101-693X
person.identifier.ridM-6101-2013
person.identifier.ridE-9360-2015
person.identifier.ridM-4223-2013
person.identifier.scopus-author-id15831018900
person.identifier.scopus-author-id56031608100
person.identifier.scopus-author-id54080259900
person.identifier.scopus-author-id55741416000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication676ea504-6aef-40d3-9b52-2074b86e1021
relation.isAuthorOfPublicationf9cfed0f-6b67-413e-988c-ac7397183471
relation.isAuthorOfPublication12c32925-de9e-4289-bdd5-1d655d7a278c
relation.isAuthorOfPublicationebcb2180-5235-4502-b562-cd1e7786e6f9
relation.isAuthorOfPublicationce3d77be-25df-4257-b5b3-aca8cff24116
relation.isAuthorOfPublicationd339a9e7-bc32-49c4-ba48-a17343d40092
relation.isAuthorOfPublication.latestForDiscoveryce3d77be-25df-4257-b5b3-aca8cff24116
relation.isProjectOfPublication221894c6-cb76-44e1-8eab-d67aa2ac373a
relation.isProjectOfPublicationa041e9a6-e6d4-4bcc-9e6f-eb9efe97a8a7
relation.isProjectOfPublication42f9a7c8-8d5c-41d5-b490-9bb0334ea269
relation.isProjectOfPublication3b77bed2-f2d5-45e7-8d6b-cd98f5f5a60d
relation.isProjectOfPublication82d48eb3-e025-4a76-9e2e-33112b8e025a
relation.isProjectOfPublicationf87227ea-f225-43b8-9138-3a1922d403a3
relation.isProjectOfPublication0c51934d-8df1-4ce5-9b6e-1d2f82ec1548
relation.isProjectOfPublication6aa7c4cc-c1e6-40d7-81e5-92b6f31c6b6f
relation.isProjectOfPublicationef3dabf9-dff0-40eb-867e-468738ac6192
relation.isProjectOfPublication.latestForDiscovery221894c6-cb76-44e1-8eab-d67aa2ac373a

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
handle9621.pdf
Size:
4.86 MB
Format:
Adobe Portable Document Format
Download

Collections

CCM2-Artigos (em revistas ou actas indexadas)
FCT2-Artigos (em revistas ou actas indexadas)