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Nanoencapsulation of Gla-Rich Protein (GRP) as a novel approach to target inflammation

2022-04-27Journal article Open access
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dc.contributor.authorViegas, Carla
dc.contributor.authorAraújo, Nuna
dc.contributor.authorCarreira, Joana
dc.contributor.authorPontes, Jorge Filipe
dc.contributor.authorMacedo, Anjos L.
dc.contributor.authorVinhas, Maurícia
dc.contributor.authorMoreira, Ana S.
dc.contributor.authorFaria, Tiago Q.
dc.contributor.authorGrenha, Ana
dc.contributor.authorde Matos, António A.
dc.contributor.authorSchurgers, Leon
dc.contributor.authorVermeer, Cees
dc.contributor.authorSimes, Dina
dc.date.accessioned2022-06-28T13:56:22Z
dc.date.available2022-06-28T13:56:22Z
dc.date.issued2022-04-27
dc.date.updated2022-05-12T19:35:56Z
dc.description.abstractChronic inflammation is a major driver of chronic inflammatory diseases (CIDs), with a tremendous impact worldwide. Besides its function as a pathological calcification inhibitor, vitamin K-dependent protein Gla-rich protein (GRP) was shown to act as an anti-inflammatory agent independently of its gamma-carboxylation status. Although GRP’s therapeutic potential has been highlighted, its low solubility at physiological pH still constitutes a major challenge for its biomedical application. In this work, we produced fluorescein-labeled chitosan-tripolyphosphate nanoparticles containing non-carboxylated GRP (ucGRP) (FCNG) via ionotropic gelation, increasing its bioavailability, stability, and anti-inflammatory potential. The results indicate the nanosized nature of FCNG with PDI and a zeta potential suitable for biomedical applications. FCNG’s anti-inflammatory activity was studied in macrophage-differentiated THP1 cells, and in primary vascular smooth muscle cells and chondrocytes, inflamed with LPS, TNFα and IL-1β, respectively. In all these in vitro human cell systems, FCNG treatments resulted in increased intra and extracellular GRP levels, and decreased pro-inflammatory responses of target cells, by decreasing pro-inflammatory cytokines and inflammation mediators. These results suggest the retained anti-inflammatory bioactivity of ucGRP in FCNG, strengthening the potential use of ucGRP as an anti-inflammatory agent with a wide spectrum of application, and opening up perspectives for its therapeutic application in CIDs.pt_PT
dc.description.sponsorshipAAC nº 41/ALG/2020—Project nº 072583—NUTRISAFE
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifierdoi: 10.3390/ijms23094813
dc.identifier.citationInternational Journal of Molecular Sciences 23 (9): 4813 (2022)pt_PT
dc.identifier.doi10.3390/ijms23094813pt_PT
dc.identifier.issn1422-0067
dc.identifier.urihttp://hdl.handle.net/10400.1/17888
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationNot Available
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relationApplied Molecular Biosciences Unit
dc.relationApplied Molecular Biosciences Unit
dc.relationInstitute for Health and Bioeconomy
dc.relationInterplay between mineralization and inflammation in vascular calcification and osteoarthritis: extracellular vesicles and marine bioactive compounds as a therapeutic approach
dc.relationStudy of the impact of dry powder insufflation over respiratory epithelial cells
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectNanoparticlespt_PT
dc.subjectGla-rich protein (GRP)pt_PT
dc.subjectChronic inflammatory diseases (CIDs)pt_PT
dc.subjectInflammationpt_PT
dc.subjectVitamin K-dependent protein (VKDP)pt_PT
dc.titleNanoencapsulation of Gla-Rich Protein (GRP) as a novel approach to target inflammationpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNot Available
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleInstitute for Health and Bioeconomy
oaire.awardTitleInterplay between mineralization and inflammation in vascular calcification and osteoarthritis: extracellular vesicles and marine bioactive compounds as a therapeutic approach
oaire.awardTitleStudy of the impact of dry powder insufflation over respiratory epithelial cells
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/DL 57%2F2016/DL 57%2F2016%2FCP1361%2FCT0006/PT
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oaire.citation.issue9pt_PT
oaire.citation.startPage4813pt_PT
oaire.citation.titleInternational Journal of Molecular Sciencespt_PT
oaire.citation.volume23pt_PT
oaire.fundingStreamDL 57/2016
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person.familyNameViegas
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person.familyNameCamilo Carreira
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person.familyNameSimes
person.givenNameCarla
person.givenNameNuna
person.givenNameJoana Sofia
person.givenNameJorge Filipe
person.givenNameMaurícia
person.givenNameAna
person.givenNameDina
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person.identifier.orcid0000-0002-6447-0242
person.identifier.orcid0000-0002-2136-1396
person.identifier.orcid0000-0002-5145-4753
person.identifier.ridN-6695-2014
person.identifier.ridH-1392-2017
person.identifier.ridN-2789-2014
person.identifier.scopus-author-id8656310300
person.identifier.scopus-author-id57195033461
person.identifier.scopus-author-id8607930100
person.identifier.scopus-author-id7201884723
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rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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