Logo do repositório
 
Publicação

Detection and mitigation of paraphysomonas sp., a chrysophycean responsible for the collapse of industrial tisochrysis lutea cultures

2026-05-14Artigo científico Acesso aberto
Ver registo simples
datacite.subject.sdg09:Indústria, Inovação e Infraestruturas
datacite.subject.sdg02:Erradicar a Fome
datacite.subject.sdg14:Proteger a Vida Marinha
dc.contributor.authorJosé, Mélissa
dc.contributor.authorPaulino, Cristina
dc.contributor.authorSchüler, Lisa
dc.contributor.authorPinto, Bruno
dc.contributor.authorCarneiro, Mariana
dc.contributor.authorRodrigues, Alexandre M. C.
dc.contributor.authorPereira, Filipe
dc.contributor.authorPereira, Hugo
dc.contributor.authorVarela, João
dc.date.accessioned2026-06-26T11:52:03Z
dc.date.available2026-06-26T11:52:03Z
dc.date.issued2026-05-14
dc.description.abstractMicroalgae are increasingly recognized as a sustainable resource to address challenges associated with climate change and population growth, owing to their capacity to produce high-quality biomass for applications in feed, food, and cosmeceuticals. However, the expansion of microalgae-based industries remains constrained by harmful biological contaminants (HBCs), which can rapidly reduce productivity, often signalled by visible changes in culture appearance (e.g., cell aggregation and discoloration), and may ultimately lead to culture collapse and substantial economic losses. In this study, the grazer Paraphysomonas sp. (Chrysophyceae) was identified as the most likely cause of Tisochrysis lutea culture collapse in a large-scale production facility using high-throughput amplicon sequencing of the 18S rDNA gene. To enable early detection of this HBC during the scale-up process, a species-specific primer pair targeting the 18S rDNA gene was developed and optimized, allowing detection of Paraphysomonas sp. at relative abundances as low as 0.1%. The contaminant was first detected at the final stage of scale-up, in a tubular photobioreactor. To further characterize the impact of the grazer and support mitigation strategies, its feeding behaviour was examined. Laboratory-scale mitigation trials showed that treatment with germanium dioxide (GeO2) at 1 mg L⁻ 1 effectively delayed contaminant proliferation and prevented culture collapse without adversely affecting T. lutea growth. These findings highlight the value of integrating molecular monitoring with targeted mitigation strategies to improve early detection and management of HBCs in industrial microalgae production systems.eng
dc.description.sponsorshipgrant 2023.01146.BDANA; UID/04033/2025
dc.identifier.doi10.1007/s10811-026-03872-2
dc.identifier.eissn1573-5176
dc.identifier.issn0921-8971
dc.identifier.urihttp://hdl.handle.net/10400.1/29154
dc.language.isoeng
dc.peerreviewedyes
dc.publisherSpringer
dc.relationAlgarve Centre for Marine Sciences
dc.relationAlgarve Centre for Marine Sciences
dc.relationCentre for Marine and Environmental Research
dc.relationUIDP/04326/2020
dc.relation.ispartofJournal of Applied Phycology
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectMicroalgae
dc.subjectIndustrial cultivation
dc.subjectHarmful biological contaminants (HBCs)
dc.subjectChrysophyceae
dc.subject18S rDNA sequencing
dc.subjectEarly detection
dc.subjectTisochrysis lutea
dc.titleDetection and mitigation of paraphysomonas sp., a chrysophycean responsible for the collapse of industrial tisochrysis lutea cultureseng
dc.typejournal article
dspace.entity.typePublication
oaire.awardNumberUIDB/04326/2020
oaire.awardNumberUIDP/04326/2020
oaire.awardNumberLA/P/0101/2020
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleAlgarve Centre for Marine Sciences
oaire.awardTitleCentre for Marine and Environmental Research
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04326%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0101%2F2020/PT
oaire.citation.endPage1832
oaire.citation.issue3
oaire.citation.startPage1819
oaire.citation.titleJournal of Applied Phycology
oaire.citation.volume38
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
person.familyNameJosé
person.familyNamePaulino
person.familyNameSchüler
person.familyNamePereira
person.familyNameVarela
person.givenNameMélissa
person.givenNameCristina
person.givenNameLisa
person.givenNameHugo
person.givenNameJoão
person.identifier.ciencia-id1C1E-B852-4086
person.identifier.ciencia-idE01F-9B43-4F24
person.identifier.orcid0000-0002-5979-0380
person.identifier.orcid0000-0001-8637-545X
person.identifier.orcid0000-0003-0961-5397
person.identifier.orcid0000-0002-1369-2099
person.identifier.orcid0000-0003-3101-693X
person.identifier.ridM-4126-2013
person.identifier.ridM-4223-2013
person.identifier.scopus-author-id55892980300
person.identifier.scopus-author-id55741416000
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isAuthorOfPublicationd2e441fe-4e8e-43e7-b4da-7484d0f5b61f
relation.isAuthorOfPublication55eb4019-c3e1-4016-8c05-f743d4af4b72
relation.isAuthorOfPublication7311726c-0c21-4c6b-bd6f-d3f01d0a5a62
relation.isAuthorOfPublicationc42792b6-4252-4c61-adaa-002b22097cce
relation.isAuthorOfPublicationd339a9e7-bc32-49c4-ba48-a17343d40092
relation.isAuthorOfPublication.latestForDiscovery7311726c-0c21-4c6b-bd6f-d3f01d0a5a62
relation.isProjectOfPublicationfafa76a6-2cd2-4a6d-a3c9-772f34d3b91f
relation.isProjectOfPublication15f91d45-e070-47d8-b6b8-efd4de31d9a8
relation.isProjectOfPublication794d4c77-c731-471e-bc96-5a41dcd3d872
relation.isProjectOfPublication.latestForDiscoveryfafa76a6-2cd2-4a6d-a3c9-772f34d3b91f

Ficheiros

Principais
A mostrar 1 - 1 de 1
Miniatura
Nome:
s10811-026-03872-2.pdf
Tamanho:
1.77 MB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
3.46 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

CCM2-Artigos (em revistas ou actas indexadas)
FCT2-Artigos (em revistas ou actas indexadas)