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Identification of molecular and physiological responses to chronic environmental challenge in an invasive species The Pacific oyster, Crassostrea gigas

2013Journal article Open access
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dc.contributor.authorClark, M. S.
dc.contributor.authorThorne, Michael A. S.
dc.contributor.authorAraújo Amaral, Ana Margarida
dc.contributor.authorVieira, Florbela A.
dc.contributor.authorBatista, Frederico
dc.contributor.authorReis, João
dc.contributor.authorPower, Deborah
dc.date.accessioned2014-10-23T10:18:02Z
dc.date.available2014-10-23T10:18:02Z
dc.date.issued2013
dc.description.abstractUnderstanding the environmental responses of an invasive species is critical in predicting how ecosystem composition may be transformed in the future, especially under climate change. In this study, Crassostrea gigas, a species well adapted to the highly variable intertidal environment, was exposed to the chronic environmental challenges of temperature (19 and 24°C) and pH (ambient seawater and a reduction of 0.4 pH units) in an extended 3-month laboratory-based study. Physiological parameters were measured (condition index, shell growth, respiration, excretion rates, O:N ratios, and ability to repair shell damage) alongside molecular analyses. Temperature was by far the most important stressor, as demonstrated by reduced condition indexes and shell growth at 24°C, with relatively little effect detected for pH. Transcriptional profiling using candidate genes and SOLiD sequencing of mantle tissue revealed that classical “stress” genes, previously reported to be upregulated under acute temperature challenges, were not significantly expressed in any of the treatments, emphasizing the different response between acute and longer term chronic stress. The transcriptional profiling also elaborated on the cellular responses underpinning the physiological results, including the identification of the PI3K/AKT/mTOR pathway as a potentially novel marker for chronic environmental challenge. This study represents a first attempt to understand the energetic consequences of cumulative thermal stress on the intertidal C. gigas which could significantly impact on coastal ecosystem biodiversity and function in the future.por
dc.description.sponsorshipThis work was supported by an EU Research Infrastructure Action under the EP7 “Capacities” Specific Programme, ASSEMBLE grant agreement no. 227799, CCMAR Ref 00415/2010. We thank Lloyd Peck (BAS) for advice on physiology and critical reading of the manuscript, Simon Morley (BAS) for statistical analyses, John Turner (BAS) for the Hadley Centre predictions, Elizabeth Harper Cambridge University for damage repair analysis, and Ana Grade (IPMA) who assisted with RNA extractions.por
dc.identifier.citationMelody S. Clark, Michael A. S. Thorne, Ana Amaral, Florbela Vieira, Frederico M. Batista, João Reis & Deborah M. Power, "Identification of molecular and physiological responses to chronic environmental challenge in an invasive species The Pacific oyster, Crassostrea gigas" in Ecology and Evolution 2013; 3(10): 3283– 3297por
dc.identifier.doihttp://dx.doi.org/10.1002/ece3.719
dc.identifier.issn2045-7758
dc.identifier.otherAUT: DPO00386;
dc.identifier.urihttp://hdl.handle.net/10400.1/5424
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherWileypor
dc.subjectCandidate genespor
dc.subjectCondition indexpor
dc.subjectEnergetic trade-offspor
dc.subjectmTOR pathwaypor
dc.subjectTranscriptional profilingpor
dc.titleIdentification of molecular and physiological responses to chronic environmental challenge in an invasive species The Pacific oyster, Crassostrea gigaspor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage3297por
oaire.citation.issue10por
oaire.citation.startPage3283por
oaire.citation.titleEcology and Evolutionpor
oaire.citation.volume3por
person.familyNameAraújo Amaral
person.familyNameBatista
person.familyNamePower
person.givenNameAna Margarida
person.givenNameFrederico
person.givenNameDeborah Mary
person.identifier.ciencia-idF61F-CD31-2466
person.identifier.ciencia-idCB1B-7DBC-6303
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0002-7663-8313
person.identifier.orcid0000-0002-2848-3011
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridM-4717-2013
person.identifier.ridF-5651-2010
person.identifier.scopus-author-id7003808684
person.identifier.scopus-author-id7101806760
rcaap.rightsopenAccesspor
rcaap.typearticlepor
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relation.isAuthorOfPublication.latestForDiscoveryd564e68d-a75e-458f-add4-bdb0739cde8d

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