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Culture media performance on the detection of actinomycetes from composts

2013Journal article Open access
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dc.contributor.authorCoelho, Luísa
dc.contributor.authorReis, Mário
dc.contributor.authorDionísio, Lídia
dc.date.accessioned2024-02-01T10:48:32Z
dc.date.available2024-02-01T10:48:32Z
dc.date.issued2013
dc.date.updated2024-01-31T16:30:22Z
dc.description.abstractIn this study we evaluated the performance of six culture media on the detection of actinomycetes that were present in composts of agro-industrial wastes from the Algarve region. The composts were produced with orange wastes, olive pomace and grass clippings, in the proportion of 2:1:1 of volume. Two compost piles were built with different ventilation systems, one with forced ventilation and the other with mechanical turning and natural ventilation. In order to quantify the population of actinomycetes, different growth media containing antibiotics were tested and one culture medium containing only half of the concentration of the Plate Count Agar (½ PCA) was also tested. The incubation took place at two temperatures, 25º and 55ºC. Different results were obtained for the enumerations from the culture media. The population of actinomycetes achieved higher values when incubated at 55ºC and when the samples were inoculated in the ½ PCA culture medium. Thus, the antibiotics showed no beneficial effect in the tested culture media. The results suggest the use of the ½ PCA culture medium as the most adequate in order to count the bacterial populations in these samples. This medium is also the less expensive and the one that showed the fastest bacterial growth.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.17660/ActaHortic.2013.1013.58pt_PT
dc.identifier.eid2-s2.0-84892778060
dc.identifier.eissn2406-6168
dc.identifier.slugcv-prod-2138738
dc.identifier.urihttp://hdl.handle.net/10400.1/20333
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherInternational Society for Horticultural Science (ISHS)pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntibioticpt_PT
dc.subjectActinomycetes growthpt_PT
dc.subjectOrangept_PT
dc.subjectOlivept_PT
dc.subjectClippingspt_PT
dc.titleCulture media performance on the detection of actinomycetes from compostspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleActa Horticulturaept_PT
oaire.citation.volume1013pt_PT
person.familyNameGuerreiro David Coelho
person.familyNameReis
person.familyNameDionísio
person.givenNameLuísa Isabel
person.givenNameMário
person.givenNameLídia
person.identifier.ciencia-id3410-D2D3-AB40
person.identifier.ciencia-idD31F-ACBA-41E7
person.identifier.orcid0000-0001-5236-4605
person.identifier.orcid0000-0002-3349-3003
person.identifier.orcid0000-0002-0530-9395
person.identifier.scopus-author-id55769965400
person.identifier.scopus-author-id6506947768
rcaap.cv.cienciaidD31F-ACBA-41E7 | Mário Manuel Ferreira dos Reis
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationd36e9efd-294f-4788-b229-c48ed3eec583
relation.isAuthorOfPublicatione3fffbf1-41db-404f-8bd5-936ab4e8f59c
relation.isAuthorOfPublicationfd39b253-0c7d-46a0-b35f-b1f682c901bc
relation.isAuthorOfPublication.latestForDiscoverye3fffbf1-41db-404f-8bd5-936ab4e8f59c

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