Publication
beta-(1,3)-glucan synthase complex from Alternaria infectoria, a rare dematiaceous human pathogen
| dc.contributor.author | Anjos, J. | |
| dc.contributor.author | Fernandes, C. | |
| dc.contributor.author | Silva, B. M. A. | |
| dc.contributor.author | Quintas, Célia | |
| dc.contributor.author | Abrunheiro, A. | |
| dc.contributor.author | Gow, N. A. R. | |
| dc.contributor.author | Gonçalves, Teresa | |
| dc.date.accessioned | 2015-12-03T14:46:01Z | |
| dc.date.available | 2015-12-03T14:46:01Z | |
| dc.date.issued | 2012 | |
| dc.description.abstract | The fungal cell wall polymer beta-(1,3)-D-glucan is synthesized by the enzyme beta-(1,3)D-glucan synthase that is a complex composed of at least two proteins, Rho1p and Fks1p. Here, we report the nucleotide sequence of a single FKS gene and of the regulatory unit, RHO1 from the dematiaceous pathogenic fungus Alternaria infectoria. The predicted AiFks and AiRho share, respectively, 93% and 100% identity with that of Drechslera tritici-repentis. We also report that the sensitivity to caspofungin of eight different A. infectoria clinical strains is similar, with a MIC > 32 mu g/ml and a MEC of 1 mu g/ml, except for one strain which had a MEC of 1.4 mu g/ml. This same strain exhibited one substitution at the hot spot 2, S1405A, compatible with less susceptible phenotypes, with the other seven strains having no mutations in either hot spot 1 or 2. The relative quantification of the expression of AiFKS and of AiRHO demonstrated a decrease in response to an exposure to caspofungin at 0.5 mu g/ml. | |
| dc.identifier.doi | https://dx.doi.org/10.3109/13693786.2012.675525 | |
| dc.identifier.issn | 1369-3786 | |
| dc.identifier.other | AUT: CQU00490; | |
| dc.identifier.uri | http://hdl.handle.net/10400.1/7303 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Informa Healthcare | |
| dc.relation.isbasedon | P-002-556 | |
| dc.title | beta-(1,3)-glucan synthase complex from Alternaria infectoria, a rare dematiaceous human pathogen | |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 725 | |
| oaire.citation.startPage | 716 | |
| oaire.citation.title | Medical Mycology | |
| oaire.citation.volume | 50 | |
| person.familyName | Quintas | |
| person.givenName | Célia | |
| person.identifier.ciencia-id | EA14-D76B-FF6E | |
| person.identifier.orcid | 0000-0001-9680-5089 | |
| rcaap.rights | restrictedAccess | |
| rcaap.type | article | |
| relation.isAuthorOfPublication | 7c06dbb4-eb11-4f22-b0f5-3cf276d919f4 | |
| relation.isAuthorOfPublication.latestForDiscovery | 7c06dbb4-eb11-4f22-b0f5-3cf276d919f4 |
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