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Piscine PTHrP regulation of calcium and phosphate transport in winter flounder renal proximal tubule primary cultures

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dc.contributor.authorGuerreiro, P. M.
dc.contributor.authorCanario, Adelino V. M.
dc.contributor.authorPower, Deborah
dc.contributor.authorRenfro, J. Larry
dc.date.accessioned2014-05-13T08:40:36Z
dc.date.available2014-05-13T08:40:36Z
dc.date.issued2010
dc.date.updated2014-05-08T10:32:23Z
dc.description.abstractMultiple factors control calcium (Ca2 ) and inorganic phosphate (Pi) transport in the fish nephron, and the recently discovered members of the piscine parathyroid hormone-like protein family are likely participants in such regulatory mechanisms. The effects of an NH2-terminal peptide (amino acids 1–34) of Takifugu rubripes parathyroid hormone-related protein, (1–34)PTHrP, on Ca2 and Pi transport were investigated in winter flounder (Pseudopleuronectes americanus) proximal tubule cells in primary culture (fPTCs). RT-PCR performed on RNA extracted from fPTCs and from intact kidney tissue indicated that expression of PTHrP and types 1 and 3 PTH/PTHrP receptors occurred both in vivo and in vitro and that circulating levels of PTHrP measured by specific radioimmunoassay averaged 2.5 0.13 ng/ml. fPTC monolayers were mounted in Ussing chambers, and under neutral electrochemical conditions, addition of 10 nM (1–34)PTHrP to the basolateral side induced a slight increase in Ca2 transport rate from luminal to peritubular side, significantly stimulating net Ca2 reabsorption. (1–34)PTHrP also significantly increased the Pi secretory flux, and slightly reduced Pi reabsorption, evoking a significant increase in Pi net secretion. This stimulatory effect was partially inhibited by bisindolylmaleimide, an inhibitor of protein kinase C. Incubation of ex vivo flounder renal tubules with (1–34)PTHrP resulted in apparent reduction of Na -Pi cotransporter type II (NaPi-II) protein in tubule membranes. PTHrP seems therefore to participate in the modulation of Ca2 and Pi homeostasis by fish kidney.por
dc.identifier.citationGuerreiro, Pedro M.; Canario, Adelino V. M.; Power, Deborah M.; Renfro, J. Larry. Piscine PTHrP regulation of calcium and phosphate transport in winter flounder renal proximal tubule primary cultures, American Journal of Physiology-Regulatory Integrative and Comparative Physiology, 299, 2, R603-R6, 2010.por
dc.identifier.doihttp://dx.doi.org/10.1152/ajpregu.00509.2009
dc.identifier.issn0363-6119
dc.identifier.otherAUT: ACA00258; DPO00386;
dc.identifier.urihttp://hdl.handle.net/10400.1/3877
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherAmerican Physiological Societypor
dc.subjectParathyroid hormone-related proteinpor
dc.subjectRenal transport;por
dc.subjectProximal tubule cellpor
dc.subjectPseudopleuronectes americanuspor
dc.titlePiscine PTHrP regulation of calcium and phosphate transport in winter flounder renal proximal tubule primary culturespor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPageR611por
oaire.citation.issue2por
oaire.citation.startPageR603por
oaire.citation.titleAmerican Journal of Physiology-Regulatory Integrative and Comparative Physiologypor
oaire.citation.volume299por
person.familyNameGuerreiro
person.familyNameCanario
person.familyNamePower
person.givenNamePedro Miguel
person.givenNameAdelino
person.givenNameDeborah Mary
person.identifierA-2539-2009
person.identifier143624
person.identifier.ciencia-id5C13-965D-3148
person.identifier.ciencia-id1F1E-D3B3-F804
person.identifier.ciencia-id891A-8A44-3CAE
person.identifier.orcid0000-0001-5371-7919
person.identifier.orcid0000-0002-6244-6468
person.identifier.orcid0000-0003-1366-0246
person.identifier.ridC-7942-2009
person.identifier.scopus-author-id56568523700
person.identifier.scopus-author-id7101806760
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublicationd0a8a148-98e7-4899-8fb0-0b8afa91e4a4
relation.isAuthorOfPublication5f6e51ee-9113-469e-8b9e-f30f2d452521
relation.isAuthorOfPublicationc68f5ffb-63f6-4c70-8957-29e464fb59c0
relation.isAuthorOfPublication.latestForDiscoveryc68f5ffb-63f6-4c70-8957-29e464fb59c0

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