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How to monitor the acclimatization of micropropagated plants - From in vitro to the field?

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dc.contributor.authorOsório, Maria Leonor
dc.contributor.authorGonçalves, Sandra
dc.contributor.authorCoelho, N.
dc.contributor.authorRomano, Anabela
dc.contributor.authorOsório, Júlio
dc.date.accessioned2015-06-18T10:12:39Z
dc.date.available2015-06-18T10:12:39Z
dc.date.issued2013
dc.description.abstractThe high mortality experienced by plants during ex vitro transplantation stage is the major bottleneck in large scale application of micropropagation. The abnormal physiological and anatomical characteristics of micropropagated plantlets require that they should be gradually acclimatized to the environment of the greenhouse or field. Environmental factors, especially relative humidity and irradiance, play a critical role in both physiological and biochemical functions, namely water relations, gas exchange, photosynthetic efficiency and water oxidation, during transition from in vitro to ex vitro conditions. Throughout this period of transition and acclimatization substantial changes in those characteristics are necessary to achieve a successful micropropagation. This adaptation should be accurately monitored using relevant physiological parameters including pigment content, chlorophyll a fluorescence imaging, net photosynthetic rate, transpiration rate, and stomatal conductance. Growth traits and oxidative stress markers, such as electrolyte leakage, lipid peroxidation and hydrogen peroxide content, should be also analyzed. These parameters must be assessed during the course of ex vitro acclimatization in several periods and compared with those of plants growing in their natural habitat. The performance plants of Tuberaria major, an endangered species endemic from the Algarve region (Portugal), during their acclimatization through indoor-to-outdoor conditions was monitored using several physiological and biochemical traits as indicators. In view of the results, we concluded that the transplantation protocol described can be used for restoration purposes, contributing to the preservation of the species. © ISHS 2013.
dc.identifier.doihttp://dx.doi.org/10.17660/ActaHortic.2013.988.5
dc.identifier.isbn9789066053991
dc.identifier.issn0567-7572
dc.identifier.otherAUT: JOS01477; ARO01015;
dc.identifier.urihttp://hdl.handle.net/10400.1/6447
dc.language.isoeng
dc.peerreviewedyes
dc.publisherInternational Society for Horticultural Science
dc.relation.isbasedonP-008-C00
dc.subjectChlorophyll a fluorescence
dc.subjectElectrolyte leakage
dc.subjectGas exchange
dc.subjectLipid peroxidation
dc.subjectTuberaria major
dc.titleHow to monitor the acclimatization of micropropagated plants - From in vitro to the field?
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F35410%2F2007/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F63501%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F31534%2F2006/PT
oaire.citation.endPage70
oaire.citation.startPage65
oaire.citation.titleActa Horticulturae
oaire.citation.volume988
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
person.familyNameOsório
person.familyNameGonçalves
person.familyNameCoelho
person.familyNameRomano
person.familyNameOsório
person.givenNameMaria Leonor
person.givenNameSandra
person.givenNameNatacha
person.givenNameAnabela
person.givenNameJúlio
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person.identifier.ciencia-idC518-BDB3-E127
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person.identifier.orcid0000-0003-4265-5622
person.identifier.orcid0000-0002-7204-7428
person.identifier.orcid0000-0002-4054-4091
person.identifier.ridM-6988-2017
person.identifier.scopus-author-id7006394339
person.identifier.scopus-author-id8583003200
person.identifier.scopus-author-id56249997600
person.identifier.scopus-author-id9938818600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccess
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