Repository logo
 
Publication

Post-transcriptional silencing of Bos taurus prion family genes and its impact on granulosa cell steroidogenesis

dc.contributor.authorPimenta, Jorge M.B.G.A.
dc.contributor.authorPires, Virgínia M.R.
dc.contributor.authorNolasco, Sofia
dc.contributor.authorCastelo-Branco, Pedro
dc.contributor.authorMarques, Carla C.
dc.contributor.authorApolónio, Joana
dc.contributor.authorAzevedo, Rita
dc.contributor.authorFernandes, Mónica T.
dc.contributor.authorLopes-da-Costa, Luís
dc.contributor.authorPrates, José
dc.contributor.authorPereira, Rosa M.L.N.
dc.date.accessioned2022-04-18T09:42:32Z
dc.date.available2022-04-18T09:42:32Z
dc.date.issued2022
dc.description.abstractPrion proteins constitute a major public health concern, which has partly overshadowed their physiological roles in several scenarios. Indeed, these proteins were implicated in male fertility but their role in female fertility is relatively less explored. This study was designed to evaluate the role of SPRN and PRNP prion family genes in bovine follicular steroidogenesis pathways. Post-transcriptional SPRN and PRNP silencing with siRNAs was established in bovine granulosa cell (GC) in vitro culture, and gene expression and progesterone and estradiol concentrations were evaluated. SPRN knockdown, led to a down regulation of CYP11A1 mRNA levels (2.1-fold), and PRNP knockdown led to an upregulation of SPRN mRNA levels (2.3-fold). CYP19A1 expression and estradiol synthesis was not detected in any experimental group. Finally, SPRN knockdown led to a mild reduction in progesterone production in GCs and this was the only experimental group that did not exhibit an increment in progesterone levels after 48 h of culture. As a conclusion, it was possible to detect the expression of the SPRN gene in bovine GCs, a potential interaction between SPRN and PRNP regulation, and the impact of SPRN expression on CYP11A1 and progesterone levels. These findings bring new insights into the role of these genes in ovarian steroidogenesis and female reproductive physiology. (c) 2022 Elsevier Inc. All rights reserved.pt_PT
dc.description.sponsorshipALT20-03-0246-FEDER000021
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.bbrc.2022.02.012pt_PT
dc.identifier.issn0006-291X
dc.identifier.urihttp://hdl.handle.net/10400.1/17758
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationCentre for Interdisciplinary Research in Animal Health
dc.subjectBovine granulosa cellspt_PT
dc.subjectPrion-like genespt_PT
dc.subjectsiRNApt_PT
dc.subjectSteroidogenesispt_PT
dc.titlePost-transcriptional silencing of Bos taurus prion family genes and its impact on granulosa cell steroidogenesispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Interdisciplinary Research in Animal Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00276%2F2020/PT
oaire.citation.endPage99pt_PT
oaire.citation.startPage95pt_PT
oaire.citation.titleBiochemical and Biophysical Research Communicationspt_PT
oaire.citation.volume598pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCastelo-Branco
person.familyNameApolónio
person.familyNameTeotónio Fernandes
person.givenNamePedro
person.givenNameJoana
person.givenNameMónica Alexandra
person.identifier.ciencia-idE015-7F8F-5CA1
person.identifier.ciencia-id791E-9E39-2795
person.identifier.ciencia-idD21D-88F0-DBD1
person.identifier.orcid0000-0002-3453-3978
person.identifier.orcid0000-0002-9727-1813
person.identifier.orcid0000-0002-1206-1367
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationbb25b5ad-1769-42be-a7d3-8fe76215aa23
relation.isAuthorOfPublication01cf2be1-ea9f-4082-bcc6-e50fe4ad9fe1
relation.isAuthorOfPublication4aeefcfb-528b-4067-95ed-fef9aacccebf
relation.isAuthorOfPublication.latestForDiscoverybb25b5ad-1769-42be-a7d3-8fe76215aa23
relation.isProjectOfPublicationf5dfda4b-8014-4249-b4d5-86803ae921b2
relation.isProjectOfPublication.latestForDiscoveryf5dfda4b-8014-4249-b4d5-86803ae921b2

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
1-s2.0-S0006291X22001942-main.pdf
Size:
350.39 KB
Format:
Adobe Portable Document Format