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Identification of the tri-Al tricitrate complex in Plantago almogravensis by hydrophilic interaction LC with parallel ICP-MS and electrospray Orbitrap MS/MS detection

dc.contributor.authorGrevenstuk, Tomás
dc.contributor.authorFlis, Paulina
dc.contributor.authorOuerdane, Laurent
dc.contributor.authorLobinski, Ryszard
dc.contributor.authorRomano, Anabela
dc.date.accessioned2015-06-18T10:12:34Z
dc.date.available2015-06-18T10:12:34Z
dc.date.issued2013
dc.description.abstractThe identification of the ligands binding Al is essential to understand the mechanisms by which plants detoxify Al internally. However, studies concerning the speciation of Al have been frustrated by its complex chemistry. This work describes the identification of the tri-Al tricitrate (Al(3)cit(3)) complex in Plantago almogravensis, encompassing an integrated mass spectrometry approach based on hydrophilic interaction liquid chromatography (HILIC) and parallel detection by ICP-MS and ESI-MS/MS. This work also reports that both Al and Fe are bound by tricitrate, sometimes simultaneously, and the consequences of this finding are discussed. Of the complexes separated by size exclusion chromatography, Al(3)cit(3) is the most stable occurring in P. almogravensis as it was the only one recovered after HILIC. This approach provided new information on the mechanism of Al detoxification in P. almogravensis, namely that Al is bound by the organic acid citrate and that the relative concentration of the detected complexes is affected by the organ type and internal Al concentration, and has potential for studying the speciation of Al in less tolerant plants.
dc.identifier.doihttp://dx.doi.org/10.1039/c3mt00101f
dc.identifier.issn1756-5901
dc.identifier.otherAUT: ARO01015;
dc.identifier.urihttp://hdl.handle.net/10400.1/6404
dc.language.isoeng
dc.peerreviewedyes
dc.publisherRoyal Society of Chemistry
dc.relation.isbasedonP-006-FV8
dc.titleIdentification of the tri-Al tricitrate complex in Plantago almogravensis by hydrophilic interaction LC with parallel ICP-MS and electrospray Orbitrap MS/MS detection
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1293
oaire.citation.startPage1285
oaire.citation.titleMetallomics
oaire.citation.volume5
person.familyNameGrevenstuk
person.familyNameRomano
person.givenNameTomás
person.givenNameAnabela
person.identifier.ciencia-idB31A-EC35-5A31
person.identifier.ciencia-id8A18-7E9A-3B0E
person.identifier.orcid0000-0003-0779-3018
person.identifier.orcid0000-0002-7204-7428
person.identifier.scopus-author-id24329026400
person.identifier.scopus-author-id56249997600
rcaap.rightsopenAccess
rcaap.typearticle
relation.isAuthorOfPublication4ae6491f-f21f-4001-8987-dec1ad73ca5c
relation.isAuthorOfPublicatione3343b60-17d3-4f5e-a2bf-06f26da69206
relation.isAuthorOfPublication.latestForDiscoverye3343b60-17d3-4f5e-a2bf-06f26da69206

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