Repository logo
 
Publication

Comparative proteomics on deep-sea amphipods after in situ copper exposure

2019Journal article Restricted access
Simple item page
dc.contributor.authorKwan, Yick Hang
dc.contributor.authorZhang, Dongsheng
dc.contributor.authorMestre, Nélia
dc.contributor.authorWong, Wai Chuen
dc.contributor.authorWang, Xiaogu
dc.contributor.authorLu, Bo
dc.contributor.authorWang, Chunsheng
dc.contributor.authorQian, Pei-Yuan
dc.contributor.authorSun, Jin
dc.date.accessioned2020-02-05T13:34:28Z
dc.date.available2020-12-01T01:30:12Z
dc.date.issued2019
dc.description.abstractThe interest in deep-sea mining increased along with the environmental concerns of these activities to the deep-sea fauna. The discovery of optimal biomarkers of deep-sea mining activities in deep-sea species is a crucial step toward the supply of important ecological information for environmental impact assessment. In this study, an in situ copper exposure experiment was performed on deep-sea scavenging amphipods. Abyssorchomene distinctus individuals were selected among all the exposed amphipods for molecular characterization. Copper concentration within the gut was assessed, followed by a tandem mass tag-based coupled with two-dimensional liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) applied to identify and quantify the protein expression changes after 48 h of exposure. 2937 proteins were identified and annotated, and 1918 proteins among all identified proteins were assigned by at least two nonambiguous peptides. The screening process was performed based on the differences in protein abundance and the specific correlation between the proteins and copper in previous studies. These differentially produced proteins include Na+/K+ ATPase, cuticle, chitinase, and proteins with unknown function. Their abundances showed correlation with copper and had high sensitivity to indicate the copper level, being here proposed as biomarker candidates for deep-sea mining activities in the future. This is a key step in the development of environmental impact assessment of deep-sea mining activities integrating ecotoxicological data.pt_PT
dc.description.sponsorshipFunding Agency National Key Research Program of China 2016YFC0304105 China Ocean Mineral Resources R&D Association Program DY135-E2-1-03 Hong Kong Branch of South Marine Science and Engineering Guangdong Laboratory Fundacao para a Ciancia e Tecnologia I.P. Portugal (FCT) Direcao-Geral de Politica do Mar (DGPM) through the project Mining-MiningImpact 2 (JPI Oceans) 2/2017/001 Portuguese Foundation for Science and Technology CEECIND005262017pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1021/acs.est.9b04503pt_PT
dc.identifier.issn0013-936X
dc.identifier.urihttp://hdl.handle.net/10400.1/13458
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherAmerican Chemical Societypt_PT
dc.subjectAnimalspt_PT
dc.subjectChromatography, liquidpt_PT
dc.subjectCopperpt_PT
dc.subjectProteomicspt_PT
dc.subjectTandem mass spectrometrypt_PT
dc.subjectAmphipodapt_PT
dc.subjectWater pollutants, chemicalpt_PT
dc.titleComparative proteomics on deep-sea amphipods after in situ copper exposurept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMAR%2F00350%2F2013/PT
oaire.citation.endPage13991pt_PT
oaire.citation.issue23pt_PT
oaire.citation.startPage13981pt_PT
oaire.citation.titleEnvironmental Science and Technologypt_PT
oaire.citation.volume53pt_PT
oaire.fundingStream5876
person.familyNameMestre
person.givenNameNélia
person.identifier195864
person.identifier.ciencia-idD71D-759D-F6FC
person.identifier.orcid0000-0001-6871-9984
person.identifier.ridC-1943-2009
person.identifier.scopus-author-id35237689600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication6ed5a7d3-57d3-4171-9aad-8c761e4cb38a
relation.isAuthorOfPublication.latestForDiscovery6ed5a7d3-57d3-4171-9aad-8c761e4cb38a
relation.isProjectOfPublication3c9090f1-c8fc-4990-8cd8-d61d72eb13f7
relation.isProjectOfPublication.latestForDiscovery3c9090f1-c8fc-4990-8cd8-d61d72eb13f7

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
acs.est.pdf
Size:
1.29 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
3.46 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

CIM2-Artigos (em revistas ou actas indexadas)