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Temperature affects methylation of the myogenin putative promoter, its expression and muscle cellularity in Senegalese sole larvae

2013Journal article Restricted access
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dc.contributor.authorCampos, C.
dc.contributor.authorValente, L. M. P.
dc.contributor.authorConceição, L. E. C.
dc.contributor.authorEngrola, S.
dc.contributor.authorFernandes, J. M. O.
dc.date.accessioned2014-07-04T09:48:27Z
dc.date.available2014-07-04T09:48:27Z
dc.date.issued2013
dc.date.updated2014-07-03T10:48:24Z
dc.descriptionTexto integral disponível através do editorpor
dc.description.abstractMyogenin (myog) encodes a highly conserved myogenic regulatory factor that is involved in terminal muscle differentiation. It has been shown in mammals that methylation of cytosines within the myog promoter plays a major role in regulating its transcription. In the present study, the Senegalese sole (Solea senegalensis) myog putative proximal promoter was identified and found to be highly conserved among teleosts. Therefore, it is plausible that it plays a similar role in controlling myog expression. Cytosine methylation of the myog promoter in skeletal muscle of Senegalese sole larvae undergoing metamorphosis was influenced by rearing temperature. A lower temperature (15°C) significantly increased myog promoter methylation in skeletal muscle, particularly at specific CpG sites, relatively to higher rearing temperatures (18 and 21°C). Myog transcription was downregulated at 15°C, whereas expression of dnmt1 and dnmt3b was upregulated, consistently with the higher myog methylation observed at this temperature. Rearing temperature also affected growth and fast muscle cellularity, producing larger fibers at 21°C. Taken together, our data provide the first evidence of an epigenetic mechanism that may be underlying the temperature-induced phenotypic plasticity of muscle growth in teleosts.por
dc.identifier.citationCampos, C.; Valente, L.M.P.; Conceição, L.E.C.; Engrola, S.; Fernandes, J.M.O.Temperature affects methylation of the myogenin putative promoter, its expression and muscle cellularity in Senegalese sole larvae, Epigenetics, 8, 4, 1-9, 2013.por
dc.identifier.doihttp://dx.doi.org/ 10.4161/epi.24178
dc.identifier.issn1559-2294
dc.identifier.urihttp://hdl.handle.net/10400.1/4652
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherEpigenetics Societypor
dc.relation.publisherversionhttps://www.landesbioscience.com/journals/epigenetics/article/24178/?nocache=1365651600por
dc.subjectSolea senegalensispor
dc.subjectEpigenetic regulationpor
dc.subjectMethylationpor
dc.subjectMyogenesispor
dc.subjectMyogeninpor
dc.subjectThermal plasticitypor
dc.titleTemperature affects methylation of the myogenin putative promoter, its expression and muscle cellularity in Senegalese sole larvaepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage397por
oaire.citation.issue4por
oaire.citation.startPage389por
oaire.citation.titleEpigeneticspor
oaire.citation.volume8por
person.familyNameEngrola
person.givenNameSofia
person.identifier237846
person.identifier.ciencia-idA610-29E7-9B83
person.identifier.orcid0000-0002-5244-5541
person.identifier.ridA-2485-2012
person.identifier.scopus-author-id8669620600
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isAuthorOfPublication51be5a20-df9c-4a90-85eb-51d779642caa
relation.isAuthorOfPublication.latestForDiscovery51be5a20-df9c-4a90-85eb-51d779642caa

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