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A new combined approach to improved lipid production using a strictly aerobic and oleaginous yeast

2019Journal article Open access
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dc.contributor.authorGuerreiro, Fábio
dc.contributor.authorConstantino, Ana
dc.contributor.authorEmília Lima-Costa, Maria
dc.contributor.authorRaposo, Sara
dc.date.accessioned2020-05-12T16:29:43Z
dc.date.available2020-05-12T16:29:43Z
dc.date.issued2019
dc.description.abstractMicrobial lipids have potential applications in energy, and food industry, because most of those lipids are triacylglycerol with long-chain fatty-acids that are comparable to conventional vegetable oils and can be obtained without arable land requirement. Rhodosporidium toruloides is a strictly aerobic strain, where oxygen plays a crucial role in growth, maintenance, and metabolite production, such as lipids and carotenoids. Dissolved oxygen concentration is one of the major factors affecting yeast physiological and biochemical characteristics. In this context, different approaches have been developed to increase available oxygen by the increasing the aeration and the addition of an oxygen-vector. The growth of R. toruloides in 2-L mechanical stirred tank reactor equipped with 1 or 2 porous spargers and a 70 C/N ratio, revealed a lipid content of 0.47 and 0.52 g/g and a lipidic productivity of 0.16 and 0.17 g/L day, respectively. The oxygen-vector addition, increased the lipidic productivity for 0.20 g/L day and a lipid contend of 0.51 g of lipids/g of biomass. The combined approach, combining high aeration (AA), and 1% of n-dodecane addition (DA), produced a significant improvement in the lipid accumulation (62%, w/w), when compared with the DA (51%, w/w) and the AA (52%, w/w) approaches. The increasing of lipids accumulation and smaller culture time are key factors for the success of scale-up and profitability of a bioprocess.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1002/elsc.201800115pt_PT
dc.identifier.issn1618-0240
dc.identifier.urihttp://hdl.handle.net/10400.1/13880
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relation.publisherversionhttps://onlinelibrary.wiley.com/doi/abs/10.1002/elsc.201800115pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBiodiesel propertiespt_PT
dc.subjectDesign reactorpt_PT
dc.subjectLipidspt_PT
dc.subjectOxygen-vectorpt_PT
dc.subjectRhodosporidium toruloidespt_PT
dc.titleA new combined approach to improved lipid production using a strictly aerobic and oleaginous yeastpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMAR%2F00350%2F2013/PT
oaire.citation.endPage56pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage47pt_PT
oaire.citation.titleEngineering in Life Sciencespt_PT
oaire.citation.volume19pt_PT
oaire.fundingStream5876
person.familyNameConstantino
person.familyNameLima Costa
person.familyNameRaposo
person.givenNameAna
person.givenNameMaria Emília
person.givenNameSara
person.identifier.ciencia-idF010-54F2-9E30
person.identifier.orcid0000-0002-9968-3512
person.identifier.orcid0000-0002-4433-5756
person.identifier.orcid0000-0001-5344-7349
person.identifier.ridL-3673-2013
person.identifier.scopus-author-id7003633118
person.identifier.scopus-author-id7801639810
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication6eb9b8a6-dd51-4ace-809b-7d7de9cdfe36
relation.isAuthorOfPublication035b3010-5f47-4700-b196-402970ff2a69
relation.isAuthorOfPublication.latestForDiscovery466ef81c-c32f-4829-a96e-97d37c157fc0
relation.isProjectOfPublication3c9090f1-c8fc-4990-8cd8-d61d72eb13f7
relation.isProjectOfPublication.latestForDiscovery3c9090f1-c8fc-4990-8cd8-d61d72eb13f7

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