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Proteomic response of gill microsomes of Crassostrea brasiliana exposed to diesel fuel water-accommodated fraction

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dc.contributor.authorMüller, Gabrielle do Amaral e Silva
dc.contributor.authorLüchmann, Karim Hahn
dc.contributor.authorRazzera, Guilherme
dc.contributor.authorToledo-Silva, Guilherme
dc.contributor.authorBebianno, Maria João
dc.contributor.authorMarques, Maria Risoleta Freire
dc.contributor.authorBainy, Afonso Celso Dias
dc.date.accessioned2020-04-07T15:09:44Z
dc.date.available2020-04-07T15:09:44Z
dc.date.issued2018
dc.description.abstractDiesel fuel water-accommodated fraction (diesel-WAF) is a complex mixture of organic compounds that may cause harmful effects to marine invertebrates. Expression of microsomal proteins can be changed by oil exposure, causing functional alterations in endoplasmic reticulum (ER). The aim of this study was to investigate changes in protein expression signatures in microsomes of oysterl Crassostrea brasiliana (=C.gasar) gill after exposure to 10% diesel-WAF for 24 and 72 h. Protein expression signatures of gills of oysters exposed to diesel-WAF were compared to those of unexposed oysters using two-dimensional electrophoresis (2-DE) to identify differentially expressed proteins. A total of 458 protein spots with molecular weights between 30-75 kDa were detected by 2-DE in six replicates of exposed oyster proteomes compared to unexposed ones. Fourteen differentially expressed proteins (six up-regulated and eight down-regulated) were identified. They are: proteins related to xenobiotic biotransformation (cytochrome P450 6 A, NADPH-cytochrome P450 reductase); cytoskeleton (α-tubulin, β-tubulin, gelsolin); processing and degradation of proteins pathways (thioredoxin domain-containing protein E3 ubiquitin-protein ligase MIB2); involved in the biosynthesis of glycolipids and glycoproteins (beta-1,3-galactosyltransferase 1); associated with stress responses (glutamate receptor 4 and 14-3-3 protein zeta, corticotropin-releasing factor-binding protein); plasmalogen biosynthesis (fatty acyl-CoA reductase 1), and sodium-and chloride-dependent glycine transporter 2 and glyoxylate reductase/hydroxypyruvate reductase. Different patterns of protein responses were observed between 24 and 72 h-exposed groups. Expression pattern of microsomal proteins provided a first insight on the potential diesel-WAF effects at protein level in microsomal fraction of oyster gills and indicated new potential biomarkers of exposure and effect. The present work can be a basis for future ecotoxicological studies in oysters aiming to elucidate the molecular mechanisms behind diesel-WAF toxicity and for environmental monitoring programs.pt_PT
dc.description.sponsorship573949/2008-5, 307467/2013-9, 573949/2008-5, 406104/2013-1pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doihttps://doi,org/10.1016/j.aquatox.2018.06.001pt_PT
dc.identifier.issn0166-445X
dc.identifier.urihttp://hdl.handle.net/10400.1/13700
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0166445X18304624pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectDiesel oilpt_PT
dc.subjectEcotoxicoproteomicspt_PT
dc.subjectMicrosomept_PT
dc.subjectCrassostrea brasilianapt_PT
dc.subjectEndoplasmic reticulumpt_PT
dc.subjectIn situpt_PT
dc.subjectCrh-bp genept_PT
dc.subjectProtein expressionpt_PT
dc.subjectBiotransformation genespt_PT
dc.subjectOysters crassostreapt_PT
dc.subjectRisk assessmentpt_PT
dc.subjectCrude oilpt_PT
dc.subjectRat liverpt_PT
dc.titleProteomic response of gill microsomes of Crassostrea brasiliana exposed to diesel fuel water-accommodated fractionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMAR%2F00350%2F2013/PT
oaire.citation.endPage118pt_PT
oaire.citation.startPage109pt_PT
oaire.citation.titleAquatic Toxicologypt_PT
oaire.citation.volume201pt_PT
oaire.fundingStream5876
person.familyNameBebianno
person.givenNameMaria
person.identifier.ciencia-id2B11-46AC-B94B
person.identifier.orcid0000-0003-1492-8566
person.identifier.scopus-author-id7004152715
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isAuthorOfPublication.latestForDiscovery2e00a26d-1dd3-4c22-a6bf-ac7943ae0d32
relation.isProjectOfPublication3c9090f1-c8fc-4990-8cd8-d61d72eb13f7
relation.isProjectOfPublication.latestForDiscovery3c9090f1-c8fc-4990-8cd8-d61d72eb13f7

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