Publication
Application of bismuth modified disposable screen printed carbon electrode for metal- plant thiols complexation studies
| datacite.subject.fos | Engenharia e Tecnologia::Outras Engenharias e Tecnologias | pt_PT |
| dc.contributor.advisor | Diaz-Cruz, Jose Manuel | |
| dc.contributor.author | Feyssa, Belachew Tolla | |
| dc.date.accessioned | 2018-07-02T10:26:37Z | |
| dc.date.available | 2018-07-02T10:26:37Z | |
| dc.date.issued | 2010-02-18 | |
| dc.date.submitted | 2010 | |
| dc.description.abstract | The application of Bismuth modified cheap and disposable screen printed carbon electrode (BiSPCE) for voltammetric studies of metal–thiol rich peptides complexation was evaluated considering systems consisting of cadmium as a metal and GSH and PC2 as thiol peptides. Comparison of the performance of BiSPCE with the commonly used glassy carbon electrode (BiFE) was made. The information obtained about the complexation sequence using the former electrode was quite consistent with previous studies made on GSH and Cd systems using the conventional mercury electrodes due to the absence of signal splitting, the good sensitivity and the wider linearity range. In contrast with the conventional mercury electrodes, the anodic signals associated with bismuth electrode material were observed to be weakened and all the available signals were well resolved which shows the suitability of Bismuth based electrode for metal- thiol complexation studies. MCR-ALS could not be applied due to the continuous shift of the peak potentials, loss of linearity of the species and anomalous shape of the complex signal formed between Cd and GSH. Therefore, the complexation sequence between GSH and Cd2+ was evaluated qualitatively. However, a relatively well defined shape and intense voltammograms were observed for Cd-PC2 system and consequently, MCR-ALS was applied after correction of the continuous peak potential shift for the complex signals. The peak intensity associated with complex signal was dominated by the intense signal of the free metal reduction in acidic and fairly basic medium. However a relatively intense signal of the complexes was obtained at pH 7.5 in borate buffer solution. In addition extensive Bi complexation was observed from ESI-MS experiment which proves the suitability of ex situ mode of Bi film preparation for thiol- metal complexation studies by voltammetric titration technique. | pt_PT |
| dc.identifier.uri | http://hdl.handle.net/10400.1/10740 | |
| dc.language.iso | eng | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Differential pulse voltammetry | pt_PT |
| dc.subject | Bismuth film electrodes | pt_PT |
| dc.subject | Screen printed carbon electrode | pt_PT |
| dc.subject | ESI-MS | pt_PT |
| dc.subject | MCR-ALS | pt_PT |
| dc.title | Application of bismuth modified disposable screen printed carbon electrode for metal- plant thiols complexation studies | pt_PT |
| dc.type | master thesis | |
| dspace.entity.type | Publication | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | masterThesis | pt_PT |
| thesis.degree.grantor | Universidade do Algarve. Faculdade de Ciências e Tecnologia | |
| thesis.degree.level | Mestre | |
| thesis.degree.name | Mestrado em Qualidade em Análises | pt_PT |