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Dietary creatine supplementation in gilthead seabream (Sparus aurata): comparative proteomics analysis on fish allergens, muscle quality, and liver

2018Journal article Open access
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dc.contributor.authorSchrama, Denise
dc.contributor.authorCerqueira, Marco
dc.contributor.authorRaposo de Magalhães, Cláudia
dc.contributor.authorRosa Da Costa, Ana
dc.contributor.authorWulff, Tune
dc.contributor.authorGonçalves, Amparo
dc.contributor.authorCamacho, Carolina
dc.contributor.authorColen, R.
dc.contributor.authorFonseca, Flávio
dc.contributor.authorRodrigues, Pedro
dc.date.accessioned2020-07-31T13:01:06Z
dc.date.available2020-07-31T13:01:06Z
dc.date.issued2018
dc.description.abstractThe quality of fish flesh depends on the skeletal muscle's energetic state and delaying energy depletion through diets supplementation could contribute to the preservation of muscle's quality traits and modulation of fish allergens. Food allergies represent a serious public health problem worldwide with fish being one of the top eight more allergenic foods. Parvalbumins, have been identified as the main fish allergen. In this study, we attempted to produce a low allergenic farmed fish with improved muscle quality in controlled artificial conditions by supplementing a commercial fish diet with different creatine percentages. The supplementation of fish diets with specific nutrients, aimed at reducing the expression of parvalbumin, can be considered of higher interest and beneficial in terms of food safety and human health. The effects of these supplemented diets on fish growth, physiological stress, fish muscle status, and parvalbumin modulation were investigated. Data from zootechnical parameters were used to evaluate fish growth, food conversion ratios and hepatosomatic index. Physiological stress responses were assessed by measuring cortisol releases and muscle quality analyzed by rigor mortis and pH. Parvalbumin, creatine, and glycogen concentrations in muscle were also determined. Comparative proteomics was used to look into changes in muscle and liver tissues at protein level. Our results suggest that the supplementation of commercial fish diets with creatine does not affect farmed fish productivity parameters, or either muscle quality. Additionally, the effect of higher concentrations of creatine supplementation revealed a minor influence in fish physiological welfare. Differences at the proteome level were detected among fish fed with different diets. Differential muscle proteins expression was identified as tropomyosins, beta enolase, and creatine kinase among others, whether in liver several proteins involved in the immune system, cellular processes, stress, and inflammation response were modulated. Regarding parvalbumin modulation, the tested creatine percentages added to the commercial diet had also no effect in the expression of this protein. The use of proteomics tools showed to be sensitive to infer about changes of the underlying molecular mechanisms regarding fish responses to external stimulus, providing a holistic and unbiased view on fish allergens and muscle quality.pt_PT
dc.description.sponsorshipProject Allyfish contract MAR 16-02-01-FMP-0014 (PEP 4107IDNAD50308.12) Mar2020pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.3389/fphys.2018.01844pt_PT
dc.identifier.issn1664-042X
dc.identifier.urihttp://hdl.handle.net/10400.1/14561
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontiers Mediapt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCreatinept_PT
dc.subjectAquaculturept_PT
dc.subjectGilthead seabreampt_PT
dc.subjectParvalbuminpt_PT
dc.subjectProteomicspt_PT
dc.titleDietary creatine supplementation in gilthead seabream (Sparus aurata): comparative proteomics analysis on fish allergens, muscle quality, and liverpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage1844pt_PT
oaire.citation.titleFrontiers in Physiologypt_PT
oaire.citation.volume9pt_PT
person.familyNameSchrama
person.familyNameCerqueira
person.familyNameRaposo de Magalhães
person.familyNameRosa da Costa
person.familyNamePontes Barbosa Colen
person.familyNameRodrigues
person.givenNameDenise
person.givenNameMarco
person.givenNameCláudia
person.givenNameAna M
person.givenNameRita Isabel
person.givenNamePedro
person.identifier159154
person.identifier305029
person.identifier.ciencia-id501C-AC69-68C0
person.identifier.ciencia-id4F1E-16FC-00D8
person.identifier.ciencia-idEB1C-0E40-F003
person.identifier.ciencia-idA418-A85E-5DB1
person.identifier.ciencia-idF113-B354-F356
person.identifier.orcid0000-0003-0274-5957
person.identifier.orcid0000-0001-7237-5053
person.identifier.orcid0000-0001-7751-2870
person.identifier.orcid0000-0003-0225-9537
person.identifier.orcid0000-0001-7104-1844
person.identifier.orcid0000-0002-9668-1204
person.identifier.ridH-6511-2016
person.identifier.ridE-2165-2012
person.identifier.ridM-3406-2013
person.identifier.scopus-author-id55755583400
person.identifier.scopus-author-id53986075100
person.identifier.scopus-author-id55323905000
person.identifier.scopus-author-id55107531000
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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